- Research article
- Open Access
Absence of glaucoma in DBA/2J mice homozygous for wild-type versions of Gpnmb and Tyrp1
BMC Genetics volume 8, Article number: 45 (2007)
The glaucomas are a common but incompletely understood group of diseases. DBA/2J mice develop a pigment liberating iris disease that ultimately causes elevated intraocular pressure (IOP) and glaucoma. We have shown previously that mutations in two genes, Gpnmb and Tyrp1, initiate the iris disease. However, mechanisms involved in the subsequent IOP elevation and optic nerve degeneration remain unclear.
Here we present new mouse strains with Gpnmb and/or Tyrp1 genes of normal function and with a DBA/2J genetic background. These strains do not develop elevated IOP or glaucoma with age.
These strains provide much needed controls for studying pathogenic mechanisms of glaucoma using DBA/2J mice. Given the involvement of Gpnmb and/or Tyrp1 in areas such as immunology and tumor development and progression, these strains are also important in other research fields.
Glaucomas are heterogeneous diseases that affect 70 million people worldwide . They are characterized by the loss of retinal ganglion cells and degeneration of the optic nerve [2, 3]. Glaucoma is often associated with an age-related elevation of intraocular pressure (IOP) . Elevated IOP induces glaucomatous neurodegeneration in susceptible individuals. IOP elevation involves compromised drainage of aqueous humor (AqH) through the ocular drainage structures in the iridocorneal angle (angle) . Nevertheless, the molecular etiology of IOP elevation varies between individuals and with the type of glaucoma. In pigmentary glaucoma, the iris is damaged resulting in dispersal of iris pigment and debris into the anterior chamber and AqH drainage structures. In susceptible individuals, the pigment and/or debris induces a process(es) that damages the drainage structures resulting in high IOP and subsequent glaucoma [6–8]. DBA/2J (D2) mice inherit a disease with similarities to human pigmentary glaucoma [9, 10], including ultrastructurally abnormal melanosomes . Around 5–6 months of age, D2 mice develop a pigment dispersing iris disease. Pigment and/or iris debris induces drainage structure damage that disrupts the outflow of aqueous humor. This causes an elevation of IOP that first occurs in a significant portion of mice around 8–9 months of age . In our colony, about 70% of D2 mice develop moderate or severe glaucoma by 12 months of age . There are now a number of independent reports on DBA/2 glaucoma including several recent ones (e.g. [10, 12–14]). Some but not all of these reports suggest earlier onset damage than we have reported. This earlier onset damage clearly does not occur in our colony as we have studied large numbers of mice . We have previously discussed possible explanations for these differences including the exact substrain used and environmental differences.
We have previously shown that the D2 iris disease is genetically separable into two distinct traits, iris pigment dispersion (IPD) and iris stromal atrophy (ISA) . IPD is characterized by a breakdown of the posterior iris pigment epithelium, slit-like transillumination and prominent pigment dispersion. IPD is caused by a mutation in the glycoprotein (transmembrane) nmb gene (GpnmbR150X), a recent mutation that arose on D2 in the early 1980's [10, 16]. ISA is characterized by deterioration of the iris stroma, and an accumulation of stromal pigment and cell debris in the drainage structures , and is caused by a recessive mutation in the tyrosinase related protein 1 gene (Tyrp1b) . D2 mice are homozygous for both GpnmbR150Xand Tyrp1b mutations and inherit a much more severe iris disease and subsequent glaucoma than mice carrying either of the two mutations alone . The published characterization of both IPD and ISA was necessarily performed using mice of a mixed genetic background when mapping and identifying the genes that underlie these traits. These phenotypes have never been fully analyzed in the context of a D2 genetic background.
D2 is one of the most widely used mouse strains in glaucoma research. Here, we describe the development of novel strains that can be used in conjunction with D2 to further our understanding of the complex mechanisms involved in pigment dispersing iris disease, IOP elevation and glaucoma. Prior to developing these strains, there were no control, age and strain-matched DBA/2J mice that did not develop these phenotypes. Control D2.B6-Tyrp1B6GpnmbB6/Sj mice (hereafter referred to as D2.Tyrp1B6GpnmbB6) are homozygous for wild type alleles of Tyrp1 and Gpnmb that are derived from strain C57BL/6J (B6). These mice do not develop any iris disease, IOP elevation or glaucoma. DBA/2J-Gpnmb+/Sj mice (hereafter referred to as D2-Gpnmb+) are homozygous for a wild-type allele of Gpnmb on a D2 genetic background. This Gpnmb+ allele is the original D2 allele that predates the R150X mutation (See Methods). These mice develop a very mild form of iris disease due to the Tyrp1bmutation, but do not develop elevated IOP or glaucoma. These strains further define the relative contributions of the Tyrp1 and Gpnmb genes to the D2 phenotype and will serve as powerful control strains in future studies of glaucoma.
We have developed control strains that will maximize the potential of D2 mice in understanding the pathogenesis of both pigment dispersing iris disease and glaucoma. For the first control strain, named D2.Tyrp1B6GpnmbB6, the mutated forms of Tyrp1 and Gpnmb were replaced with wild-type alleles from B6 (Tyrp1B6and GpnmbB6, see Methods). Mutations in Tyrp1 alter coat color in various strains . As a consequence of the Tyrp1B6allele, D2.Tyrp1B6GpnmbB6mice have a darker coat color than D2 mice (Figure 1a). The extent of B6-derived sequence flanking both Tyrp1 and Gpnmb genes in this strain was assessed using polymorphic markers. For Tyrp1B6, the proximal breakpoint was mapped to between D4Mit151 and D4Mit178 and the distal breakpoint to between D4Mit185 and D4Mit146 (Figure 1b). The maximum size of the B6-derived region flanking Tyrp1B6is 50 Mb or 1.9% of the mouse genome. For GpnmbB6, the proximal breakpoint was mapped to between D6Mit268 and D6Mit207, and the distal breakpoint to between D6Mit277 and D6Mit16 (Figure 1c). The maximum amount of B6 sequence flanking GpnmbB6is estimated to be 36 Mb or 1.4% of the mouse genome.
D2.Tyrp1B6GpnmbB6show no iris disease, IOP elevation or glaucoma
As previously reported, the irides of D2 mice with both the GpnmbR150Xand Tyrp1bmutations develop severe pigment dispersion and iris atrophy (Figure 2a–c). However, as expected, the irides of D2.Tyrp1B6GpnmbB6mice appear totally normal at all ages. IOP elevation is a key risk factor for glaucoma and occurs in D2 mice as a result of the iris disease. Typically, a population of D2 mice shows elevated IOP levels that peak at around 9–10 months of age (Figure 3a). In contrast, IOPs of D2.Tyrp1B6GpnmbB6mice are not elevated compared to young mice at any assessed age (Figure 3b).
D2.Tyrp1B6GpnmbB6mice were further assessed for optic nerve damage. The amount of RGC axon damage was determined using a three point damage rating system based on the amount of axon damage detected in the retrorbital portion of the optic nerve . This system has been validated against axon counting [18, 19]. 70% of D2 mice have glaucomatous optic nerve damage by 12 months of age (Figure 3c). In contrast, D2.Tyrp1B6GpnmbB6show no evidence of glaucomatous damage at 12 months of age (Figure 3d).
D2.Tyrp1B6GpnmbB6mice have B6-derived chromosomal regions (congenic intervals) flanking Tyrp1 and Gpnmb respectively. Together, these B6-derived regions represent up to 3.3% of the genome (Figure 1). To produce an even more closely matched control strain, we developed D2-Gpnmb+, a second control strain that has no B6-derived congenic intervals. To generate the D2-Gpnmb+ strain, the GpnmbR150Xallele of modern day D2 mice was replaced with the Gpnmb+ allele from the D2-sdy strain (the original wild-type D2 Gpnmb allele, see Methods).
D2-Gpnmb+ mice develop a mild iris disease
D2-Gpnmb+ mice were aged and assessed for ISA and IPD. Although wild-type for Gpnmb, D2-Gpnmb+ mice are homozygous for the Tyrp1bmutation. Therefore, they are expected to develop the ISA iris disease that is caused by this mutation. A mild ISA disease is observed in these mice (Figure 4a–c). The ISA phenotype in D2-Gpnmb+ mice is characterized by a change in iris stromal morphology (appearing "roughened" and lacking complexity). However, the iris shows no evidence of IPD, maintains integrity, and is overall very mildly affected when compared to the severe, combined ISA and IPD phenotype of D2 mice.
D2-Gpnmb+ mice do not develop elevated IOP or glaucoma
IOP was assessed at a variety of ages between 4 months and 23 months of age. The selected ages are relevant to the window of IOP changes observed in DBA/2J mice. There was no age-related increase of IOP in these mice. Additionally, no D2-Gpnmb+ mice had IOP values above 21 mmHg, levels considered to be glaucoma-suspect in people (Figure 4d). Consistent with this, we detected no signs of glaucomatous RGC death and optic nerve degeneration in D2-Gpnmb+ mice. D2 mice show RGC death, loss of the nerve fiber layer and severe optic nerve cupping by 12 months of age . Retina and optic nerve morphology was examined in 4 eyes taken from D2-Gpnmb+ mice at each of 4.5 months, 12 months and 18 months of age (Figure 5a–c). The RGC layer and nerve fiber layer was normal, and there was no evidence of optic nerve head cupping (Figure 5d). Optic nerve damage was further assessed by sectioning a portion of the retro-orbital optic nerve and staining with PPD, a stain that sensitively detects degenerating axons. There was no evidence of glaucomatous optic nerve damage in D2-Gpnmb+ eyes taken from a large number of mice between 4.5 and 15 months of age (Figure 5e–i). Importantly, there was no correlation between optic nerve damage and measured IOP level in individual mice and thus the range of pressure seen in D2-Gpnmb+ did not affect nerve damage. In addition, we compared axon number in young pre-glaucomatous D2 eyes with aged D2-Gpnmb+ eyes, using a previously described counting system [18, 19], and there was no significant difference in axon number (51,748 ± 907 for D2, and 52,074 ± 427 for D2-Gpnmb+, n = 10 for each group, p = 0.7). A moderate form of optic nerve damage was present in one eye from a 12 months old mouse, and 2 eyes from mice over 16 months of age. This may be a very rare case of glaucoma due to the mutant Tyrp1 gene, or more likely age-related damage that is not related to glaucoma as a similar degree of damage is observed in other non-glaucomatous mouse strains of a similar age [12, 20].
DBA/2J is an established mouse model for inherited glaucoma that shows iris pigment dispersion, IOP elevation and optic nerve degeneration. D2 mice are homozygous for both the GpnmbR150Xand Tyrp1b mutations. Both of these mutations are necessary for the severe iris pigment disease that leads to IOP elevation and glaucoma . Although DBA/2J is an experimentally tractable model of glaucoma, it is an inbred mouse strain and so no strain matched DBA/2J controls without glaucoma have been available. Here we describe the generation of two derivative strains of DBA/2J (D2.Tyrp1B6GpnmbB6and D2-Gpnmb+), where either the mutant alleles of both Gpnmb and Tyrp1 or only Gpnmb have been replaced by wild-type versions. D2.Tyrp1B6GpnmbB6mice contain a maximum of 3.3% of B6-derived sequence on a D2 background. In the case of D2-Gpnmb+, the only known difference compared to D2 is a single base change in the Gpnmb gene. Neither D2.Tyrp1B6GpnmbB6nor D2-Gpnmb+ strains develop IOP elevation or glaucoma.
Since determining that the Gpnmb mutation recently arose on the DBA/2J background , we have produced a further control strain, D2.Tyrp1B6Gpnmb+, that is currently being characterized. This strain contains a congenic interval flanking Tyrp1 but is improved over D2.Tyrp1B6GpnmbB6in that it lacks a congenic interval around Gpnmb (as the Gpnmb+ allele is the ancestral DBA/2J allele). This strain is wild-type for both glaucoma inducing genes and is not expected to develop any D2 glaucoma-associated phenotypes. Although characterization of large numbers of these mice is a lengthy undertaking, initial analyses agree with the findings reported here for D2.Tyrp1B6GpnmbB6mice, with no evidence of iris disease, IOP elevation or glaucoma.
Regarding the control strains, the following points are pertinent. Although they lack all iris disease and glaucoma, the D2.Tyrp1B6GpnmbB6strain does not typically reproduce as well as the D2-Gpnmb+ strain and appears to have an increased incidence of early deaths (based on retrospective observations, but no specific spontaneous death study has been conducted). Although not well characterized, the decreased fecundity appears to be related to the Tyrp1B6 allele on a D2 background, since other D2 strains that we have been producing with this allele have exhibited a similar phenotype. These issues do not prevent the use of this strain. However, since the D2-Gpnmb+ strain lacks these issues and has no B6 derived interval, we recommend it as a control when studying glaucomatous neurodegeneration. Although the D2-Gpnmb+ strain still develops a mild iris disease, glaucomatous nerve damage was clearly absent in the vast majority, if not all, of these mice. Thus, despite the spread in IOP, the highest IOPs were not significant enough to induce glaucomatous axon loss. When using these control strains, it is important to remember that they may still have some undefined but glaucoma relevant phenotype(s).
It is clear that glaucoma is a multifactorial disease and D2 mice are likely to have susceptibility factors in addition to those associated with the Gpnmb and Tyrp1 genes. Thus, although these strains do not develop glaucoma, as defined by the absence of nerve damage, they may still have other phenotypes that render the D2 background susceptible to glaucoma. Such phenotypes may or may not have contributed to nerve the damage detected in a small percentage of mice.
Tyrp1 and Gpnmb function in melanosomes, organelles that produce pigment. Manifestation of both IPD and ISA in D2 eyes is dependent upon active pigment production [16, 20]. The mechanisms involved in IPD and ISA and the subsequent IOP elevation are complex and in addition to pigment production appear to involve immunity [21, 22]. Ongoing functional mouse genetics experiments complemented with a variety of immunological, molecular and cellular techniques are vital to unravel these mechanisms. The control strains described here will be a valuable resource in elucidating the biological mechanisms involved in pigment-related iris disease. In addition to ocular-related diseases, these strains provide an important resource to test the roles of Tyrp1 and Gpnmb in other diseases. Tyrp1 is the most common melanoma antigen and Gpnmb is used as a marker for metastatic melanomas  and has recently been identified as a potential molecular therapeutic target in patients with glioblastoma multiforme . Gpnmb may also be important in antigen presenting cells that control immune responses .
To improve our understanding of glaucoma, it is essential that genes and pathways involved in IOP elevation and glaucomatous RGC and optic nerve degeneration are identified. By analyzing gene expression profiles in D2 mice at various ages , and conducting subsequent functional tests, it is possible to identify differentially expressed genes important for the onset and/or progression of glaucoma. Individual mouse strains, such as D2, have a unique collection of alleles affecting many biological processes and that affect gene expression levels and protein activity . Therefore, many gene expression differences are observed between D2 and other naturally occurring strains that do not get glaucoma . This can complicate experiments and make it difficult to prioritize genes for subsequent functional testing. Additionally, it is possible that strain differences can mask some expression changes that occur in D2 mice and are relevant to the glaucoma. By enabling analysis of age- and strain-matched control mice, the control strains reported here alleviate the difficulties of controlling experiments using D2 mice.
The strains describes here will enable the systematic evaluation of the processes involved in iris disease, IOP elevation and glaucoma, work that is likely to provide novel understanding and lead to targets for improved therapeutics for human glaucomas.
Animal husbandry and strain development
Mice were housed in a 14 h light to 10 h dark cycle under previously described conditions [9, 15]. The Jackson Laboratory's (Bar Harbor, Maine, United States) pathogen surveillance program regularly screened for pathogens. All experiments were conducted in accordance with the Association for Research in Vision and Ophthalmology's statement on the use of animals in ophthalmic research and were approved by our institutional animal care and use committee. Modern DBA/2J (D2) mice (#000671, see ) have mutations in both Tyrp1 and Gpnmb. Although the majority of included data for modern D2 mice has been previously published, over 300 modern D2 mice were aged and analyzed over the same period of time as the mice of the other strains presented here. For the generation of the D2.Tyrp1B6GpnmbB6strain, D2 mice were crossed to C57BL/6J (B6) to create F1s. Progeny carrying the B6 allele of both Gpnmb (GpnmbB6) and Tyrp1 (Tyrp1B6) were then backcrossed to D2 for ten generations. Brother/sister matings were then established to generate mice homozygous for GpnmbB6and Tyrp1B6and to maintain a stable D2.Tyrp1B6GpnmbB6doubly homozygous colony.
The GpnmbR150Xmutation arose in the early 1980s and became fixed in the ancestors of modern day D2 mice . The sdy mutation alters coat color and occurred in DBA/2J (D2) mice in 1983. At that point, the DBA/2J-Dtnbp1sdystrain (hereafter referred to as D2-sdy) was separated from the main D2 colony. Genotyping a D2-sdy colony for Gpnmb in the early 2000s revealed that these mice had an original wild-type allele of Gpnmb (Gpnmb+). To develop the D2-Gpnmb+ strain, D2-sdy were crossed to modern D2 mice for three generations. We are continuing to backcross D2-Gpnmb+ to modern day D2 to further reduce the possibility of D2-Gpnmb+ mice harboring unknown genetic differences compared to modern day D2 mice. These higher generation D2-Gpnmb+ mice will be provided to the community. (They are being accepted for distribution by Jackson Laboratory mouse resources and will become strain DBA/2J-Gpnmb+/SjJ with stock # 007048). However, to hasten characterization of mice with a D2 genetic background and a wild type allele of Gpnmb, brother/sister matings that did not carry the sdy mutation were selected to establish the D2-Gpnmb+ strain that is homozygous for the wild-type Gpnmb allele and characterized here. These matings also produced the mice for clinical examinations, IOP measurements and assessment of glaucomatous damage. Analysis of 102 microsatellite markers (average spacing 13.25 cM) revealed no allelic differences between D2-Gpnmb+ and modern day D2.
The GpnmbR150Xmutation creates a novel Pvu II enzyme site . DNA isolated from tail snips was used to PCR amplify the 125 bp region surrounding the mutation using primers nmb7 (CTACAACTGGACTGCAGGGG) and nmb8 (AGCTCCATTTCTTCCATCCA). The resulting product was digested with Pvu II (NEB). The presence of the mutation is indicated by the presence of two bands of 50 bp and 75 bp. Identification of B6-derived sequence that included Tyrp1 on Chromosome 4 was achieved using the flanking polymorphic markers D4Mit178 (D2 = 170 bp, B6 = 146 bp) and D4Mit327 (D2 = 92 bp, B6 = 106 bp). Identification of B6-derived sequence that included Gpnmb on Chromosome 6 was achieved using the flanking polymorphic markers D6Mit74 (D2 = 150 bp, B6 = 160 bp) and D6Mit355 (D2 = 106 bp, B6 = 124 bp). The extent of the B6-derived region was analyzed using polymorphic markers spanning Chromosomes 4 and 6 (see ).
We examined eyes at 2–24 months of age with a slit-lamp biomicroscope (Haag-Streit) and photographed them with a 40× objective lens. Phenotypic assessment of iris stromal atrophy, dispersed pigment and transillumination was carried out as described previously [9, 15, 20].
IOP and optic nerve assessment
Mice were acclimatized to the procedure room, anesthetized by using an intraperitoneal injection of ketamine/xylazine mixture and IOP measured as previously described . Optic nerve cross sections were examined for glaucomatous damage using a modified paraphenylenediamine (PPD) staining protocol to stain the myelin sheath of all axons and the axoplasma of damaged axons as described previously . Optic nerves were assessed for glaucomatous damage using a non-biased three point grading system that has been validated against axon counting [18, 19]. Axon counts were carried out as previously described [18, 19]. 10 randomly selected D2 eyes (pre-glaucomatous, 3 to 4 months) and 10 randomly selected D2-Gpnmb+ eyes (no glaucoma, 10.5 to 11 mos) were counted.
Eyes were fixed in 4% paraformaldehyde (PFA) overnight at 4°C and stored in 0.4% PFA at 4°C. For general morphology, eyes were processed, embedded in plastic, sectioned and stained with Hemotoxylin and Eosin.
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The authors thank Amy Snow for strain development and maintenance, and IOP measurements. We thank Greg Peterson and the John Lab for technical assistance. We also thank The Jackson Laboratory Scientific Services including Pete Finger and Barbara Mortimer for technical assistance. Scientific support services at The Jackson Laboratory are subsidized by a core grant from the National Cancer Institute (CA34196). This work was supported in part by the National Eye institute, EY11721 (S.W.M.J), F32EY014515 (R.T.L.) and F32EY07015 (M.G.A.). S.W.M. John is an Investigator of The Howard Hughes Medical Institute.
GH carried out optic nerve assessment of strains, coordinated data collection and analysis and prepared the manuscript. RL participated in the design of the experiment, carried out optic nerve assessment of strains and helped write the manuscript. JKM performed the axon counts. LW organized the generation of the strains, IOP measurements and harvesting. IC carried out the IOP measurements. RS carried out optic nerve assessment. MA participated in the design of the experiment and strain generation, carried out clinical assessments of the mice, and helped write the manuscript. SJ conceived the study and participated in its design and coordination and oversaw all components including manuscript preparation. All authors read and approved the final manuscript.
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Howell, G.R., Libby, R.T., Marchant, J.K. et al. Absence of glaucoma in DBA/2J mice homozygous for wild-type versions of Gpnmb and Tyrp1. BMC Genet 8, 45 (2007). https://doi.org/10.1186/1471-2156-8-45
- Optic Nerve
- Glaucomatous Damage
- Optic Nerve Damage
- Pigmentary Glaucoma