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Table 3 Alignment of RNA-Seq derived MHC class I alleles with a genomic de novo assembly from the same individual for three animals

From: Uncovering novel MHC alleles from RNA-Seq data: expanding the spectrum of MHC class I alleles in sheep

Rambouillet (BENZ2616) accession SAMN17575729; assembly GCF_016772045.1

allel

exon 2

intron 2

exon 3

 

start

End

identity

length [bp]

start

end

identity

LfL2082

27177386

27177657

100%

196

27177853

27178129

100%

LfL2083

27748,145

27747874

100%

199

27747675

27747399

100%

LfL2084

27664922

27664651

89%

197

27664454

27664178

91%

LfL2085

27707857

27707586

93%

200

27707386

27707110

93%

LfL2086

27707857

27707586

91%

200

27,707,386

27707110

92%

LfL2087

26,983,972

26984243

100%

194

26984437

26984713

100%

LfL2088

27664922

27664651

100%

197

27664454

27664178

100%

LfL2089

27707857

27707586

100%

200

27707386

27707110

100%

LfL2090

27707857

27707586

99%

200

27707386

27707110

98%

HuSheep accession SAMN13678651; assembly ASM1117029v1

allel

exon 2

intron 2

exon 3

 

start

End

identity

length [bp]

start

end

identity

LfL2091

30275282

30275011

100%

193

30274818

30274542

100%

LfL2092

30347015

30346744

100%

199

30346545

30346269

100%

LfL2093

30204669

30204397

100%

201

30204196

30203919

100%

LfL2094

29123944

29124215

94%

271

29123944

29124215

95%

LfL2015

29705916

29706187

100%

196

29706383

29706659

100%

LfL2095

29421461

29421732

96%

195

29421927

29422203

89%

LfL2096

20406404

20406675

100%

221

20406896

20407172

100%

Ovis ammon polii x Ovis ariesaccession SAMN26012028 assembly GCA_023701675.1

allel

exon 2

intron 2

exon 3

 

start

End

identity

length [bp]

start

end

identity

LfL2097

34975557

34975828

100%

195

34976023

34976300

100%

LfL2098

35749893

35749622

90%

197

35749425

35749149

90%

LfL2099

35749893

35749622

91%

197

35749425

35749149

91%

LfL2100

35786189

35785918

100%

195

35785723

35785447

100%

LfL2101

35019521

35019791

100%

186

35019977

35020255

100%

LfL2102

35786189

35785918

95%

195

35785723

35785555

92%

LfL2103

35855847

35855576

100%

194

35855382

35855106

100%

LfL2104

35855847

35855576

89%

194

35855382

35855106

95%

LfL2105

34975557

34975828

94%

271

34975557

34975828

94%

  1. Results from alignment of RNA-Seq derived MHC alleles against genomic assembly for animal BENZ2616, the Huheep and the Ovis ammon polii x Ovis aries cross. Exon 2 and 3 had been aligned separately, the start and end position according to the assembly numbering and the length of the intron are indicated