Fig. 8

(A) Unsupervised clustering reveals segregation of IL-6 with complement genes. The heat map was generated from all-time samples of the 3 cases of COVID-19 (Case 1 - severe illness, Cases 2, 3 - moderate illness) and log (gene expression) of four cytokine genes (IL-6, IL-8, TNFα, IL1β) and genes functional in NETosis (including complement factors) [13]. Unsupervised hierarchical clustering reveals segregation of IL-6 with the complement factors C3, C5 and CFB while the other 3 cytokines (IL-8, TNFα, IL1β) were assigned to a separate clade. The samples are arranged according to the case IDs (from left to right: Case 1, Case 2, Case 3), with increasing days post-onset of illness from left to right. Gene expression data were extracted from [13]. (B) Temporal Line plot IL6 vs NETosis genes in severe COVID-19. These line plots show the temporal relation of other important cytokines along with IL-6