Fig. 4

pDC activation increases and decreases chromatin accessibility of thousands of regions. A Pearson correlation plot for samples used in ATAC-Seq. pDCs (CD3⁻CD19⁻CD11c⁺CD11b^lowB220⁺SiglecH⁺CD317⁺) were sorted from BM-derived Flt3-L cultures of C57BL/6 Nmice and cells were left either naïve or stimulated with CpG for 2 h (n = 2). B Genomic location distribution of open chromatin sites in naïve and CpG stimulated pDCs according to ATAC-Seq. Two biological replicates were used per condition, and results are shown for pooled samples per condition. C Number of differentially accessible peaks detected using DESeq2, comparing naïve to 2 h CpG stimulated pDCs, |FC| > 2 and p < 0.05. D Heatmap of normalized ATAC-Seq peak intensities (log₂FC relative to the mean for each peak). Limited to peaks (16,607) that are condition-dependent with |FC| > 2 and p < 0.05 for at least one pairwise comparison of interest. E Differential motif analysis for cluster I and II from (D) using MEME Centrimo and the HOCOMOCO v11 motif database. Significant motifs were categorized into known TF families for visualization and interpretation