Table 4 Primers for 5′unknown sequences
From: Cloning and expression analysis of GATA1 gene in Carassius auratus red var
Primer name | Sequence (5′ → 3′) | Application |
|---|---|---|
GATA1-SP1 | CAGAGCAAGGCTGTGGAAGTCATTT | 5′- Walking PCR amplification |
GATA1-SP2 | GTCCTGGTTTGGAGGTTGTTTGCC | Â |
GATA1-SP3 | GCTTCCACCTTTGATAGAGGCTGA | Â |
GATA1-F3 | ATGGCTGTAGTGCTCATTCATCGCT | verification |
GATA1-R3 | CAAGAGATTCACAACTATGACTGCG | Â |