Fig. 2From: The effects of the DNA Demethylating reagent, 5-azacytidine on SMCHD1 genomic localizationSMCHD1 occupancy over chromatin regions containing DUX4 gene on chromosome 4. a An illustration representing SMCHD1 peaks in SH-SY5Y cells without any treatment (blue) and in cells exposed to 5-azaC (red). b Protein levels of SMCHD1 in SH-SY5Y and SMCHD1 sgRNA knockout cells (KO). Lysine specific demethylase 1 (LSD1) was used as an internal control for loading. c mRNA quantification of DUX4 in SH-SY5Y (control) and SMCHD1 KO cells (KO). The copy numbers are relative to beta-actin cDNA levelsBack to article page