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Fig. 3 | BMC Genomic Data

Fig. 3

From: A simple method to estimate the in-house limit of detection for genetic mutations with low allele frequencies in whole-exome sequencing analysis by next-generation sequencing

Fig. 3

Correlation of allele frequencies validated by WES and droplet digital PCR (ddPCR) analysis. The degree of correlation of the 20 genetic mutations detected in the reference material across quadruplicate technical replicate experiments (Expt. 1–4) was assessed by linear regression analysis. The combination (ddPCR-AFs vs. WES-AFs) for regression analysis is displayed in each sequencing data size of 5 (a), 15 (b), 30 (c), and 40 (d) Gbp. In the graphs, each dot color represents one of the four technical replicates. e The relationships between experimentally detected WES-AFs and pre-validated ddPCR-AFs were verified by Pearson’s correlation coefficient (r). The p-value of the correlation was determined using the t-distribution. Mean values [± standard deviation (SD)] of the r and p-values obtained from four experiments were calculated for each sequencing data size (*P < 0.05)

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BMC Genomic Data

ISSN: 2730-6844

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