Transferability, development of simple sequence repeat (SSR) markers and application to the analysis of genetic diversity and population structure of the African fan palm (Borassus aethiopum Mart.) in Benin

Table 7 AMOVA results

Analysis	Scale	df	SS	MS	Est. var.	% Total variance	P value
Non-hierarchical AMOVA	Among regions	2	309.407	154.704	1.944	24%	< 0.001
	Among sites	6	254.302	42.384	1.903	23%	< 0.001
	Within sites	171	739.100	4.322	4.322	53%	< 0.001
	Total	179	1302.809		8.169	100%
Hierarchical AMOVA, K = 2	Among regions	1	66.765	66.765	0.205	7%
	Among sites	7	212.421	30.346	0.703	24%
	Among individuals	171	379.675	2.220	0.195	7%
	Within individuals	180	329.500	1.831	1.831	62%
	Total	359	988.361		2.933	100%
	*F-statistics*	*Value*	*P-value*
	Fst	0.310	0.010
	Fis	0.096	0.010
	Fit	0.376	0.010
Hierarchical AMOVA, K = 3	Among regions	2	152.676	76.338	0.478	16%
	Among sites	6	126.510	21.085	0.472	16%
	Among individuals	171	379.675	2.220	0.195	7%
	Within individuals	180	329.500	1.831	1.831	62%
	Total	359	988.361		2.975	100%
	*F-statistics*	*Value*	*P-value*
	Fst	0.319	0.010
	Fis	0.096	0.010
	Fit	0.385	0.010

df degree of freedom, SS sum of squares, MS mean squares, Est. var. estimated variance, Fst inter-sites genetic differentiation, Fis genetic differentiation of individuals within sites, Fit differentiation of individuals from the total

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