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Table 2 Summary of crosses. Crosses with more than 20 seeds were defined as successful, with 1–20 seeds as difficult and without seeds as not successful

From: Targeted generation of polyploids in Hydrangea macrophylla through cross-based breeding

Cross type Seed bearer Pollinizer Number of crosses Crossing success Germination rate [%] Number of F1 plants analyzed by flow cytometry Description of the F1 population regarding ploidy
selfings
(n = 6)
Baby Blue   1 not successful
Bela   3 not successful
Benelux   1 not successful
Enziandom   2 not successful
Hörnli   1 not successful
R.F. Felton   1 not successful
diploid x diploid
(n = 9)
Dark Angel Paris 3 successful 76 60 58 diploids, 2 triploids
Paris Dark Angel 1 successful n.d. 424a 105 diploids, 317 triploids, 2 aneuploids
Dark Angel Sheila 4 successful 52 54 54 diploids
Sheila Dark Angel 4 successful 47 68 34 diploids, 34 triploids
Dark Angel Hörnli 4 not successful
Hörnli Dark Angel 4 successful 81 60 19 diploids, 40 triploids, 1 tetraploid
Hörnli Sheila 3 successful 45 60 60 diploids
Sheila Hörnli 5 successful 64 34 2 diploids, 31 triploids, 1 tetraploid
Sweet Dreams Bläuling 7 successful 46 70a 69 diploids, 1 triploid
diploid x triploid
(n = 7)
Baby Blue Bela 1 difficult n.d. 4 aneuploids between 2x and 3x level
Baby Blue Enziandom 2 difficult n.d. 1 aneuploid between 2x and 3x level
Bodensee Bela 4 successful 60 61 aneuploids between 2x and 3x level
Choco Bleu Zorro 4 successful 32 26 aneuploids between 2x and 3x level
Libelle Enziandom 5 successful 59 76 aneuploids between 2x and 3x level
Little Prince Enziandom 4 successful 41 35 aneuploids between 2x and 3x level
Sweet Dreams Blaumeise 1 successful 64 14a aneuploids between 2x and 4x level
triploid x diploid
(n = 9)
Bela Baby Blue 1 not successful
Bela Bodensee 5 successful 42 63 aneuploids between 2x and 3x level
Blaumeise Sweet Dreams 1 successful 42 24a aneuploids between 2x and 4x level
Enziandom Baby Blue 1 successful n.d. 37 aneuploids between 2x and 3x level
Enziandom Libelle 3 successful 28 35 aneuploids between 2x and 3x level
Enziandom Little Prince 3 successful 44 42 aneuploids between 2x and 3x level
Oregon Pride Choco Bleu 1 difficult n.d. 3 aneuploids between 2x and 3x level
R.F. Felton Baby Blue 2 successful 47 59 aneuploids between 2x and 3x level
Zorro Choco Bleu 5 successful 82 110 aneuploids between 2x and 4x level
triploid x triploid
(n = 8)
Bela Enziandom 4 not successful
Enziandom Bela 3 difficult n.d. 3 n.d.
Bela R.F. Felton 1 not successful
R.F. Felton Bela 1 not successful
Enziandom R.F. Felton 1 not successful
R.F. Felton Enziandom 1 not successful
Enziandom Zorro 4 difficult n.d. 10 aneuploids between 2x and 4x level
Zorro Enziandom 3 not successful
diploid x tetraploid
(n = 3)
Baby Blue Benelux 1 not successful
Bodensee Benelux 4 successful 46 83 triploids
Little Prince Benelux 1 not successful
tetraploid x diploid
(n = 4)
Benelux Baby Blue 1 not successful
Benelux Bodensee 5 successful 54 60 triploids
Benelux Forever Pink 1 successful n.d. 58 triploids
Benelux Little Prince 1 difficult n.d. 1 triploid
triploid x tetraploid
(n = 2)
Bela Benelux 4 successful 34 192 aneuploids between 3x and 4x level
Enziandom Benelux 3 difficult n.d. 4 aneuploids between 2x and 4x level
tetraploid x triploid
(n = 2)
Benelux Bela 1 successful n.d. 136 aneuploids between 3x and 4x level
Benelux Enziandom 2 difficult n.d. 1 aneuploid between 3x and 4x level
  1. aData presented already in Tränkner et al. [12, 14], n.d. not determined