Table 1 Primers used for qRT-PCR and generating dsRNAs. Primer sequences for RpL19 and actin3 are described in [28]. Lowercase letters correspond to the T7 sequence
From: Anosmic flies: what Orco silencing does to olive fruit flies
|  | Primer | Primer sequence (5’> 3′) |
|---|---|---|
For qRT-PCR | c57709_g1_Orco_F3 | CTTCTTTGGCGAGAGTGTGA |
c57709_g1_Orco_R3 | GGCATTCCACGGATAGAAAGA | |
Rpl19_F | AACAAACGTGTACTGATGG | |
Rpl19_R | CACGTACTTTATGTCGTCTG | |
actin3_F | CCACCAGAACGTAAATACTC | |
actin3_R | TCTCATTGAGCGTTTAGAAG | |
For dsRNA synthesis | c57709_Orco_1_F | taatacgactcactatagggCAATGAGGAGAAGGAACCCA |
c57709_Orco_1_R | taatacgactcactatagggCAGTGAAGAACTTCGCTCCC | |
GFP-F | taatacgactcactatagggCCGCCAGTGTGCTGGAA | |
GFP-R | taatacgactcactatagggGATATCTGCAGAATTCGCC |