Genetic diversity and population structure analyses of Plectranthus edulis (Vatke) Agnew collections from diverse agro-ecologies in Ethiopia using newly developed EST-SSRs marker system

Table 3 Informativeness and levels of different diversity indices of the SSR loci across populations

SSR loci	MAF	NA	Ne	GD	Ar	Arp	Ho	He	F_ST	Nm	PIC	I	P_HWE^a	F
PE_01	0.87	7	1.35	0.24	2.91	0.15	0.22	0.23	0.08	2.90	0.23	0.45	0.530^ns	0.11
PE_02	0.49	6	2.24	0.55	3.05	0.13	0.97	0.55	0.01	35.07	0.45	0.87	0.000*	−0.75
PE_03	0.87	8	1.34	0.25	3.32	0.06	0.19	0.23	0.04	5.47	0.24	0.49	0.000*	0.23
PE_04	0.91	2	1.19	0.16	2.02	0.07	0.18	0.16	0.06	4.07	0.15	0.28	0.112^ns	−0.07
PE_05	0.79	10	1.56	0.36	3.56	0.18	0.27	0.35	0.04	5.52	0.34	0.66	0.000*	0.27
PE_06	0.46	9	3.17	0.70	4.97	0.08	0.86	0.68	0.03	8.54	0.66	1.32	0.000*	−0.22
PE_07	0.97	9	1.06	0.05	1.72	0.46	0.01	0.05	0.04	5.67	0.05	0.12	0.783^ns	0.73
PE_08	0.96	5	1.09	0.08	1.68	0.20	0.07	0.08	0.05	4.66	0.08	0.15	0.115^ns	0.18
PE_09	0.68	6	2.04	0.50	4.28	0.08	0.38	0.49	0.03	7.47	0.47	0.95	0.000*	0.25
PE_10	0.72	5	1.70	0.42	2.43	0.11	0.54	0.40	0.05	5.23	0.34	0.62	0.000*	−0.29
PE_11	0.77	6	1.66	0.38	3.11	0.16	0.39	0.37	0.03	7.64	0.35	0.68	0.335^ns	−0.01
PE_12	0.76	7	1.65	0.39	3.40	0.17	0.29	0.37	0.05	5.22	0.36	0.70	0.000*	0.26
PE_13	0.96	4	1.09	0.08	2.00	0.09	0.04	0.08	0.02	11.76	0.07	0.17	0.542^ns	0.53
PE_14	0.81	4	1.44	0.30	2.24	0.07	0.30	0.28	0.09	2.69	0.26	0.46	0.965^ns	0.04
PE_15	0.90	5	1.24	0.19	2.66	0.08	0.13	0.18	0.05	4.56	0.18	0.36	0.876^ns	0.30
PE_16	0.70	12	1.80	0.46	4.03	0.30	0.49	0.43	0.06	3.70	0.42	0.81	0.115^ns	−0.06
PE_17	0.63	6	1.99	0.51	2.90	0.06	0.57	0.48	0.06	3.87	0.44	0.78	0.000*	− 0.10
PE_18	0.68	8	1.96	0.49	3.40	0.26	0.43	0.48	0.03	8.65	0.44	0.85	0.128^ns	0.11
PE_19	0.53	5	2.12	0.53	2.87	0.07	0.88	0.53	0.01	25.01	0.43	0.82	0.000*	−0.65
PE_20	0.72	4	1.65	0.41	2.22	0.07	0.54	0.38	0.06	4.11	0.33	0.59	0.000*	−0.33
Mean	0.76	6.45	1.67	0.35	2.94	0.14	0.39	0.34	0.04	5.84	0.32	0.61		−0.09

MAF Major allele frequency, NA Number of alleles, Ne Effective number of alleles, GD Gene diversity, Ar Allelic richness, Arp Private allelic richness, H_O Observed heterozygosity, H_e Expected heterozygosity, Fst Inbreeding coefficient within subpopulations relative to total (genetic differentiation among subpopulations), Nm Gene flow estimated from Fst 0.25(1- Fst)/Fst, PIC Polymorphic information content, I Shannon’s Information Index, F Fixation Index, P_HWE^a P-value for deviation from Hardy Weinberg equilibrium, ns not significant, * = P < 0.0001 and hence highly significant

ISSN: 2730-6844