Table 2 Primers for site-directed mutagenesis
From: Regulatory single nucleotide polymorphisms (rSNPs) at the promoters 1A and 1B of the human APC gene
SNP | Mutagenic oligonucleotide primer 5’- > 3’ |
|---|---|
rs115658307: C > T | Forward:GGAGGGTAAGTAGCAAGGGGGCGG Reverse:GCTACTTACCCTCCGCCATCTTGTGGG |
rs138386816: C > T | Forward:GGCCGCTGGAAGCCTAGCCGCTGCT Reverse:GCTTCCAGCGGCCACACCCCGCCC |
rs79896135: C > G | Forward:GCTGCTGGGGGGGGACCTGCGGGCT Reverse:CCCCCCCAGCAGCGGCTAGGCTTCC |
rs78429131:T > G | Forward:AAGCACGCAGTTGCCTTCTCGGGC Reverse:CAACTGCGTGCTTCACCTTCCTCA |
rs75612255: T > C | Forward:CTCTCCCCCCCACCTCCGGCAT Reverse:AGGTGGGGGGGAGAGTAATAAATTA |
rs113017087: T > C | Forward:GCTGGACGCGGACCAGGGCGCTCCCC Reverse:GTCCGCGTCCAGCGGATTACACAGC |