Development of chromosomal markers based on next-generation sequencing: the B chromosome of the cichlid fish Astatotilapia latifasciata as a model

Table 1 Primers for qualitative PCR markers

Primer ID	Primer sequence
49B1-F	5′ GAGCTTCACACTTGCAGAGGTAAGTCATTTTTGCAGAGAC 3′
49B2-F	5′ GCTTCACACTTGCAGAGGTAAGTCATTTTT 3′
49C + −F	5′ GTTTACAGTCTGATGATGGGACATCATGCTCTGC 3′
49-R	5′ TGTCCAGAGTATAATCGCAGCCTTTGCGGT 3′
80B-F	5′ GAGGCATTACATCGGTCTTTCCATCA 3′
80C + −F	5′ GGTGAGCAGCAGGATTTTGAATTGAATGCG 3′
80-R	5′ CCTGATTGAGTGCTTCTCACAC 3′
182B1-F	5′ GGGTGTGTTTGGTTGTGGTTTGACAAGGAGTG 3′
182B2-F	5′ GGAGTGAATTGTGATGGT 3′
182B3-F	5′ GGTTTGACAAGGAGTGAATTGTGATGGTTAGATC 3′
182B4-F	5′ GAGTGAATTGTGATGGTTAGATCACTAGGTAT 3′
182C + −F	5′ AGAATGGTCCAAGGAAGG 3′
182-R	5′ CCATCAGAACCAGCATTAA 3′
207B-F	5′ GAGACACTTCTTGGAGAAAATGAAATGCCCAC 3′
207C + −F	5′ ACCAGGCCAGGAGACGACTGAAGAACT 3′
207-R	5′ GACCTGCAGAAATGTGAACATGGTTGCAGTTTACAA 3′
323B-F	5′ GGGGGTGTTTTGCTTTTGGTTTTCCTACATTAGTTA 3′
323C + −F	5′ GTATAAGCCATCTCTGTCATCTAAGGTACA 3′
323-R	5′ GACACAGTACAGCTGACACAGACGAAGCAACAG 3′
764B-F	5′ CCTGAGATGGTCCGATTGGGCTGGTAA 3′
764C + −F	5′ GGTGAAGCATCAAAGAGCTCTCTGAGTCT 3′
764-R	5′ GGAGACAAGGAGATGCGTGTTGGTGAAGTCCTAA 3′
1100B-F	5′ GGGTGTGTGGAGATGTACATCAGCACACATGTT 3′
1100C + −F	5′ CACTGAGACGGCATTGGCATGAGAAA 3′
1100-R	5′ AGCATGGTGGCAGAGGTCTTTA 3′
1987B-F	5′ CCCTCCTGTTATTCATTCCCTA 3′
1987C + −F	5′ TACTTTGCTGTGTGTTTTGCCTGTC 3′
1987-R	5′ AAGTGTGGCTGTGTGCAGGCAGGAAT 3′
2519B-F	5′ GCAGGATTCAGGAGTGAAGCATCTGTGTGA 3′
2519C + −F	5′ CACTAAACTGCAGACATCAGGCTG 3′
2519-R	5′ CATTGTTCTGCTGCAGTCAATGGAC 3′

The numbers 49, 80, 182, 207, 323, 1100 and 1987 are references to the contigs obtained with 454 sequencing data
The letters B and C indicate the specificity of the primer as a B-specific primer or positive control (B or C+), respectively, followed by the reverse/forward annotation (F or R)

ISSN: 2730-6844