Table 1 List of primer pairs, used for amplification of alliinase gene from Allium sativum L.

FWD-ALL_total

5´ F TAATTAGCTATGGTGGAGTCT

63 °C

REV-ALL_total

3´ R ATCAGACCGAGACGGCCT

63 °C

FWD-ALL_part1

5´ 1F TAATTAGCTATGGTGGAGTCTTACA

65 °C

REV-ALL_part1

3´ 1R GACCCCTACTCCAAACACAAT

65 °C

FWD-ALL_part2

5´ 2F AGCACAAGGAAGCCAGTGCAG

65 °C

REV-ALL_part2

3´ 2R TGGTGCCTTTCTGCGTTTTCA

65 °C

FWD-ALL_part3

5´ 3F GATACGTGTGGGCCGGAAATG

65 °C

REV-ALL_part3

3´ 3R AATGAAAGGAACGACGGGAGGC

65 °C