Table 2 Primer sequences used in strain generation, verification, sequencing
Name | Used for | 5′ - 3′ sequence |
|---|---|---|
kanMX4::LEU2 - fwd | Replace genomic kanMX4 with LEU2 (from pACT2) | GCTGCAGGTCGACGGATCCCCGGGTTAAT TAAGGCGCGCCAGATCAACTGTGGGAATACTCAGGT |
kanMX4::LEU2 - rev | Replace genomic kanMX4 with LEU2 (from pACT2) | TTTATTGTCAGTACTGATTAGAAAAACTCATCGAGCATC AAATGATCATGATTTTCTGTTACACC |
LEU2 – fwd | Downtag amplification and sequencing in double mutants | AGGTGTAACAGAAAATCATGAT |
pFA6 – fwd | Downtag sequencing primer (kanMX4) | CTCGACATCATCTGCCCAGA |
pFA6 – rev | Uptag sequencing primer (kanMX4) | GTCTGCAGCGAGGAGCCGTA |
Uptag | Barcode forward primer | GATGTCCACGAGGTCTCT |
Downtag | Barcode reverse primer | CGGTGTCGGTCTCGTAG |
Tal1::HPH1-fwd | Replace genomic TAL1 with hphMX4 (from pAG32) | TAGTAAAATACTTCTCGAACTCGTCACATATACGTGTACATAAGCTTGCCTTGTCCCCGCCG |
Tal1::HPH1-rev | Replace genomic TAL1 with hphMX4 (from pAG32) | GCATAAGGACATGGCCTAAATTAATATTTCCGAGATACTTCCTCGACACTGGATGGCGGCGT |
NQM1::NTC-fwd | Replace genomic NQM1 with natMX4 (from pAG25) | CCATCTAGAATGGGGTGGACAACATATAAAAGAAGAG |
NQM1::NTC-rev | Replace genomic NQM1 with natMX4 (from pAG25) | TACGTCAGAATTTTAATGAATATATAAGTCTGTACAC |