First description of the complete human xylosyltransferase-I promoter region

BMC Genomic Data

Table 1 Primers used for amplification, sequencing and site-directed mutagenesis (SDM) of XYLT1 promoter fragments

Application		Primer sequence ( 5′ → 3 ′)	T _A[°C]
PCR and sequencing	Fragment A	CCCTGTTTCGCGGCCCCTG	Slowdown-PCR
PCR and sequencing	Fragment A	CCGGAGTCGAGGCTGCTGAA	Slowdown-PCR
	Fragment B1	ATGGATGGGGAAAAGGACAC	58.8
	Fragment B1	TGGGGGAGGAGCCGAGGGAG	58.8
	Fragment B2	CAGTCAGGATGGGAAAGAAC	58.8
	Fragment B2	GGTGCCAACGATGTACTAAG	58.8
	Fragment C	AACAATCTCTTCCCACTCCC	61.2
	Fragment C	GGAGTTACTCAACCTTCGCA	61.2
	Fragment D	CATGCCCGGCTAATTTTTGT	61.2
	Fragment D	CTTCGCATCTTGTCTGCTGT	61.2
	Fragment XYLT1 _238*	ACAGGGGTGTGGGGAGGGGGCGCCGCGCGGGCCAGGCGCC	58.8
	Fragment XYLT1 _238*	GCTCGGGCCGCCGCCGCCGCCGCCGCCTCGGCTCGCCGCT	58.8
Plasmid sequencing		CTAGCAAAATAGGCTGTCCC
		CTTAATGTTTTTGGCATCTTCCA
SDM	c.-403C>T	TCCCCCCGGCGCCTTCCCCATTACCCTCCCCTCCAGCGGGGA
		TCCCCGCTGGAGGGGAGGGTAATGGGGAAGGCGCCGGGGGGA
	c.-1088C>A	GCTGGGAGGCTGCGGGGCCAGACTTTGGGGCTTGCATCCTGC
		GCAGGATGCAAGCCCCAAAGTCTGGCCCCGCAGCCTCCCAGC

ISSN: 2730-6844