Table 3 Primers used in the amplification of the whole bovine CD14 gene and other PCR conditions

BoCD14.83330F

5'ATT ACC TTC TTC TGC ACC TCC A 3'

2.5 mM

1578

BoCD14.84907R

5' GGC AGC CTC TGA GAG TTT ATG T 3'

BoCD14.84746F

5' CTT CCT GTT ATA GCC CCT TTC C 3'

2.5 mM

832

BoCD14.85577R

5' CAC GAT ACG TTA CGG AGA CTG A 3'

BoCD14.85456F

5' GGG TAC TCT CGT CTC AAG GAA C 3'

2.0 mM

825

BoCD14.86280R

5' CTG AGC CAA TTC ATT CCT CTT C 3'

BoCD14.86081F

5' ACC TGA CTC TGG ACG GAA ATC 3'

2.0 mM

747

BoCD14.86827R

5' TAC AGG AGA GCA ACC CTG AAA 3'