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Table 4 Immune responses (mean ± SD) and analyses of variance in the group immunized with Mycobacterium butyricum

From: Correlated effects of selection for immunity in White Leghorn chicken lines on natural antibodies and specific antibody responses to KLH and M. butyricum

   Line
Measure Sample size ND3-L PHA-L CC-L Control
Trait under selection
   ND3 48 6.10 ± 1.85a 2.60 ± 1.22b 1.75 ± 1.26c 2.68 ± 1.36b
   PHA 48 1.20 ± 0.86b 1.87 ± 0.68a 1.33 ± 0.53b 1.43 ± 0.74b
   CC 48    0.33 ± 0.08a 0.17 ± 0.04b
Before immunization with M.B. (d 0)
   M.B. 50 6.27 ± 1.67a 5.07 ± 1.47b 4.64 ± 1.55b 5.13 ± 1.13b
   LPS 50 1.57 ± 1.54 1.45 ± 1.49 1.31 ± 1.65 2.08 ± 1.71
   LTA 50 5.14 ± 1.68a 4.46 ± 1.43b 3.92 ± 1.70b 4.17 ± 1.75b
   Anti-Gal 50 11.96 ± 2.98b 10.81 ± 2.68b 13.72 ± 5.81a 11.61 ± 3.17b
After immunization with M.B.(d 11)
   M.B. 50 7.73 ± 1.69a 7.19 ± 1.34a 6.29 ± 1.46b 6.50 ± 1.07b
   LPS 49 2.53 ± 1.54a 2.26 ± 1.36ab 1.73 ± 1.65b 2.77 ± 1.82a
   LTA 48 6.44 ± 1.54a 5.91 ± 1.17ab 5.55 ± 1.51b 5.51 ± 1.58b
   Anti-Gal 50 16.48 ± 3.55a 14.60 ± 2.66b 13.72 ± 3.79bc 12.82 ± 3.02c
   Analysis of variance
   Line Sex Interaction R 2
Trait under selection
   ND3 48 *** ns ns 0.84
   PHA 48 ** ns ns 0.71
   CC 48 *** ns ns 0.78
Before immunization with M.B. (d 0)
   M.B. 50 *** *** ns 0.68
   LPS 50 ns ns ns 0.53
   LTA 50 * ns ns 0.69
   Anti-Gal 50 ** *** *** 0.82
After immunization with M.B. (d 11)
   M.B. 50 *** ns ns 0.61
   LPS 49 * ns ns 0.57
   LTA 48 * ns ns 0.68
   Anti-Gal 50 *** *** ns 0.77
Responsiveness (d 11-d 0)
   M.B. 50 * *** ns 0.72
   LPS 50 ns * ns 0.61
   LTA 50 ns ** ns 0.69
   Anti-Gal 50 *** *** *** 0.96
  1. a-c: Means in the same row with no common superscript differed significantly (P ≤ 0.05) based on Duncan's multiple range test.
  2. 1 Antibody titers for to M.B., LPS and LTA are the log2 of the reciprocal of the antibody dilution.
  3. 2Antibody titers to anti-Gal epitope are measured in plasma samples as the agglutination titer of glutaraldehyde stabilized rabbit. Titers are expressed as the double of the column number of the last plasma dilution showing clear evidence of agglutination.
  4. Responsiveness, measured as difference between the Ab titer at day 0 and day 11 post M. butyricum immunization.
  5. ND3-L = line selected for high antibody response to ND3 3 wk after vaccination.
  6. PHA-L = line selected for high cell-mediated immune response, to PHA at 9 wk of age.
  7. CC-L = line selected for high phagocytic activity, measured as carbon clearance at 12 wk of age.
  8. Control = line selected at random
  9. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.