Figure 3

Bone marrow genotype affects glaucomatous IOP elevation. Plot of IOP vs Gpnmb genotype, with age and numbers of mice indicated. D2-Gpnmb⁺ mice exhibit an average IOP typical of many non-glaucomatous mouse strains. As previously described, D2 mice (homozygous for the Gpnmb^{R 150X}mutation) exhibit an elevated IOP at 10 mo of age, as do D2 mice receiving syngeneic bone marrow transfers (R150X → R150X). Chimeric mice in which D2-Gpnmb⁺ bone marrow has been transferred into D2 hosts (WT → R150X) continue to maintain non-glaucomatous IOP values, whether examined at 10 mo, or beyond. The IOPs of both Gpnmb⁺ mice and chimeric mice with Gpnmb⁺ bone marrow were significantly lower than those of all mice with Gpnmb^{R 150X}mutant marrow (P < 0.002 for all comparisons at various ages, t test).