Figure 2

A P. pacificus mbd-2 mutant is viable and Egg laying defective. A The genomic locus of Ppa-mbd-2. The gene is part of an operon and is transspliced to SL2. The first gene of the operon, mapmodulin, is a microtubule binding protein. MnCo-epimerase: Methyl-malonyl CoA epimerase. B Ppa-mbd-2 gene structure. Ppa-mbd-2 is composed of six exons. Highlighted in blue are the exons removed in the deletion allele tu365 that cause the fusion of exon 1 and 6 and result in a frame shift. C Agarose gel showing the genomic amplification of the Ppa-mbd-2 gene with the primers AG10334–AG10335, indicating a deletion of approx. 1.7 kb in tu365. D Ppa-mbd-2(tu365) animals are egg-laying defective and form a "bag of worms" phenotype (arrow). See wild type animals for comparison.