Skip to main content

Table 6 PCR Primer sequences, length of PCR products and similarity with known genes

From: Gibberellins and heterosis of plant height in wheat (Triticum aestivum L.)

Genes primer sequences length of PCR product (bp) Similarities with homologous genes
Actin S 5'-TCATTGGTATGGAAGCTGCTGAATC-3'
A 5'-CCTGACTCATCATACTCGCCCTTCG-3'
300 Oryza sativa actin mRNA,
complete cds
Identities = 279/330 (93%)
Actin S 5'-GGAAGTACAGTGTCTGGATTGGAGGGT-3'
A 5'-TTCAGAAGACCCAGACAACTCGCAAC-3'
150 Oryza sativa actin mRNA,
complete cds
Identities = 140/150 (93%)
14-3-3 S 5'- CACTATGTCTGGGGTCG -3'
A 5'- ATTTAGGACTTGCTGGCA -3'
340 Triticum aestivum 14-3-3
protein Identities = 100%
RSG S 5'-TGGGCTACCGGACTACGCCAAG-3'
A 5'-CCT TGGAACTTGACCTGCCGCTT-3'
367 Nicotiana tabacum mRNA for
bZIP transcriptional
activator RSG Identities = 67%
KAO S 5'-ACAACTGCCTGGCCAAGATCACCAG-3'
A 5'-GTGACAACTTTGACTCATCCGCGACAACAA-3
184 Hordeum vulgare KAO mRNA
Identities = 166/184(90%)
KS S 5' CCCCTGAACTTTCTGATGCTTGCATAT-3'
A 5'-TTGAAGGACTGTACTTCTCAACCAATGCT-3'
137 Hordeum vulgare KS-like gene
Identities = 116/127 (91%)
TaGA2ox-1 S 5'-TCGCTCCGCCTAAGCCACAG-3'
A 5'-CTGCAACTCAAGCAGTCATCCCTC-3'
104 Oryza sativa mRNA for
OsGA2ox1
Identities = 73/104 (71%)
TaGA20ox-2 S 5'-GCTGAGCCAGGGCGTGGAGAAG-3'
A 5'-CCATGAAGGTGTCGCCGATGTTG-3'
260 Oryza sativa gibberellin
20-oxidase 2 (Sd-1) gene
Identities = 240/260 (92%)
TaGA20ox1D S 5'-AGCACTACCGGGCGGACATGAA-3'
A 5'-GCCATCCATCCATGCTTCTTCGTAC-3
260 Wheat GA20-oxidase 1 gene
homoeologous TaGA20ox1D
Identities = 100%
TaGA3ox2-1 S 5'-GTACATGGGCGTGCGCAAGAA G-3'
A 5'-GCACGCATCCACCAGCATCATC-3'
219 Wheat GA3-oxidase 2 gene
homoeologous TaGA3ox2-1
Identities = 100%
TaGA3ox2-2 S 5'-GTACATGGGCGTGCGCAAGAAG-3'
A 5'-CAGCTAAGCTACCAGCCCACCATG-3'
258 Wheat GA3-oxidase 2 gene
homoeologous TaGA3ox2-2
Identities = 100%
TaGA3ox2-3 S 5'-GTACATGGGCGTGCGCAAGAAG-3'
A 5'-GCTAATCTAACAGCCCGCCACCAT-3'
260 Wheat GA3-oxidase 2 gene
homoeologous TsGA3ox2-3
Identities = 100%
GAI S 5'-GCACATTCCTGGACCGCTTCACC-3'
A 5'-GCAGCCTTCCTTCTCCTCCACCTTG-3'
422 Triticum aestivum Rht-1 gene
Identities = 100%
TaGID1 S 5'-CCACCATCGGCTTCTACCTGCTGTC-3'
A 5'-GGCGAGCTCATCCACGACGAGAC-3'
133 Oryza sativa mRNA for GID1
Identities = 120/133 (90%)
GAMYB S 5'-CGTGAGAAGTTCAAGTTCCTCTGT-3'
A 5'-AAGTTTTCAGGATGAGACGAAGTG-3'
287 Triticum aestivum MYB3 gene
Identities = 100%
GIP S 5'-GGGACGCAGTACAAGAAGG-3'
A 5'-GGAAACTGGGAGGGCAAT-3'
300 Petunia hybrida mRNA for (gip5 gene)
Identities = 64/69 (92%)
XET S 5'-GCCCTTCGTCGCCTCCTAC-3'
A 5'-CGGCACAACAACAACTAGTGGTAG-3'
300 Wheat mRNA for endo-
xyloglucan transferase
Identities = 100%