Plasmid name | Primer position | Primer Sequence 5'-3' | Number of 5' codons | Number of 3' codons |
---|---|---|---|---|
pBBΔorc1 | 5' forward | CGCAAGCTTGACTCCACCCTTCCGAGAGT | 3 | 2 |
 | 5' reverse | CGCACTAGTGGTGATCATGGGTTTGCGTC |  |  |
 | 3' forward | CGCACTAGTCGAAACTAGCTCTCCAAGCTC |  |  |
 | 3' reverse | CGCGGATCCTCTACTGTACAGCAGATGAG |  |  |
pBBΔorc2 | 5' forward | CGCAAGCTTCAACAAAATTATGCGTAGAG | 2 | 3 |
 | 5' reverse | CGCACTAGTCGTCATTGAATATCACACGG |  |  |
 | 3' forward | CGCACTAGTGCCACGTTCTGAGTATTCTG |  |  |
 | 3' reverse | CGCGGATCCGATCTTGTACTTGTCCTCGC |  |  |
pBBΔorc3 | 5' forward | CGCGGATCCTGAAACGGGTCTGTGAGTGG | 4 | 6 |
 | 5' reverse | CGCACTAGTCCCTTTCACCATCTCGATAA |  |  |
 | 3' forward | CGCACTAGTCAGACGTTGATGGGATGGTGA |  |  |
 | 3' reverse | CGCGAATTCGGTGAGGACCTCGAGTTCGAT |  |  |
pBBΔorc4 | 5' forward | CGCAAGCTTCAGGAGACAGCTACCTACGA | 3 | 2 |
 | 5' reverse | CGCACTAGTGGGCGTCATTGAATATCACA |  |  |
 | 3' forward | CGCACTAGTCGCGAGTAATGACCCCTACT |  |  |
 | 3' reverse | CGCGGATCCTCGGCTATCAAGGGTTCAGC |  |  |
pBBΔorc5 | 5' forward | CGCAAGCTTAGTTGGTGACGCTCATCGGC | 4 | 2 |
 | 5' reverse | CGCGAATTCGCGACCAGCCATACGTATGC |  |  |
 | 3' forward | CGCGAATTCGAGGAGTAGTTGCCAGGCGA |  |  |
 | 3' reverse | CGCGGATCCTGATCGAGGCGAACACGCTG |  |  |
pBBΔorc6 | 5' forward | CCGGAATTCTTCGAGGCGACGCTGCGGGA | 7 | 6 |
 | 5' reverse | CTCTTCGGGGTCCTCATCCAT |  |  |
 | 3' forward | GCGGTCCTGGAGCGCCTGTAG |  |  |
 | 3' reverse | CCGGAATTCCAGCGCCTCAACCCGATCGAC |  |  |
pBBΔorc7 | 5' forward | CGCGGATCCGCGCCCGAACGCAACTAGAA | 3 | 3 |
 | 5' reverse | GCGCTCGAGGTCTGTCATGTATTCACGCAC |  |  |
 | 3' forward | GCGCTCGAGGAGAACAATTAGTGGATGCT |  |  |
 | 3' reverse | GCGAAGCTTGGTGTGATGTTCATGACCAT |  |  |
pBBΔorc8 | 5' forward | CCGGAATTCCACGTCGTGTTGGCGGTGGT | 3 | 3 |
 | 5' reverse | GCGCTCGAGCGTCTTCATCGCTTGCCGAGA |  |  |
 | 3' forward | GCGCTCGAGGCGACCGTGTAGACCCCGGA |  |  |
 | 3' reverse | CGCAAGCTTGATGAAGCTCCGCCGCAGCG |  |  |
pBBΔorc9 | 5' forward | CGCAAGCTTTGGGTCGTGTACACGGCCTC | 4 | 3 |
 | 5' reverse | CGCACTAGTGCGGCAGGTCATATAAGAGT |  |  |
 | 3' forward | CGCACTAGTGTAACCCAATAAGCTGCGAA |  |  |
 | 3' reverse | CGCGGATCCAACACTCATCGACGAGTGAA |  |  |
pBBΔorc10 | 5' forward | CCGGATCCCCTGTGGTCGTTCTGGAAGAC | 5 | 2 |
 | 5' reverse | CCACTAGTACCCAACAGTGACATCCTCC |  |  |
 | 3' forward | CGCACTAGTGTGCTGTAGCGATTGTGCGA |  |  |
 | 3' reverse | CGCCTCGAGCACGGAGACGTCGAGAGCGAG |  |  |
pBBΔpolD1 | 5' forward | CCGGAATTCGTCGGTCGGATCGGGGACAT | 2 | 2 |
 | 5' reverse | GCGCTCGAGCGTCATGTCCCCGTCGATCT |  |  |
 | 3' forward | GCGCTCGAGTTCTCGTAGCCACGGCGGCG |  |  |
 | 3' reverse | GCGAAGCTTCGTTGTGCACCATCGTCACC |  |  |
pBBΔpolD2 | 5' forward | GCGGAATTCGGCGTGGCGGTAACGGCGTT | 1 | 2 |
 | 5' reverse | GCGACTAGTCATTACAGCCAGCGGTCGAG |  |  |
 | 3' forward | GCGACTAGTTTCATGTGACCACGGCGCTC |  |  |
 | 3' reverse | GCGAAGCTTGACGTGATCGACGAACACACC |  |  |
pBBΔpolB1 | 5' forward | CCGGAATTCGCCAACACTGCCGCGTTGAA | 3 | 3 |
 | 5' reverse | CGCACTAGTGTTTCCCATTGGGTTCGGGT |  |  |
 | 3' forward | CGCACTAGTCAGTTCACGTAGCCCGCTGG |  |  |
 | 3' reverse | CGCAAGCTTCTCGTCAACAACGCCGGGCT |  |  |
pBBΔpolB2 | 5' forward | CGCGAATTCCCGAACGACGCGGCATCAAG | 2 | 2 |
 | 5' reverse | CGCACTAGTCGGCATTCCCTACAGAACCA |  |  |
 | 3' forward | CGCACTAGTCTGTCGTAGTGGACCCCACC |  |  |
 | 3' reverse | CGCAAGCTTCGGCTTTTCCTGGCCAAGTC |  |  |
pBBΔmcm | 5' forward | CGCGAATTCGTCGAGAACCCCAGGATGAG | 3 | 3 |
 | 5' reverse | CGCACTAGTCGGATCCATCTGGTAGAGAT |  |  |
 | 3' forward | CGCACTAGTCGCTCGATCTAGCCGACGGC |  |  |
 | 3' reverse | CGCAAGCTTACAGCACGCCCACGTGCTCGT |  |  |
pBBΔpri1 | 5' forward | ATGAGTCCCCCACTCGGTCTT | 2 | 6 |
 | 5' reverse | GTGCATGCCGGCAATCGTGG |  |  |
 | 3' forward | GCCGAGAAGGCCACAGAATGA |  |  |
 | 3' reverse | GGCGGCTCTTCGACCTGACT |  |  |
pBBΔpri2 | 5' forward | TCTGTCAGGACCGGGCCACT | 2 | 2 |
 | 5' reverse | GTTCATGCTGGCCCGTGTTTG |  |  |
 | 3' forward | CCCGATTAGCCCTGCTTGCC |  |  |
 | 3' reverse | ATGGCTAACTCCAACGCCAA |  |  |
pBBΔpcn | 5' forward | TCTCGTCTGCGGCGGGGGTA | 4 | 4 |
 | 5' reverse | CGCCTTGAACATTATTGCAGA |  |  |
 | 3' forward | ATCCAGTCCAACTGACGCCA |  |  |
 | 3' reverse | ATGCTGGCCCGTGTTTGCGA |  |  |
pBBΔrad2 | 5' forward | CCGGAATTCGTGATGTCGAACACGGGGAA | 3 | 2 |
 | 5' reverse | CGCCTCGAGGTTCCCCATCACGCCCAGTT |  |  |
 | 3' forward | CGCCTCGAGTGGACGTGACCGGCGCTGAT |  |  |
 | 3' reverse | CGCAAGCTTGCGTAAAAGCCATCGGAACC |  |  |
pBBpolD1 all | 5' forward | CGCGAATTCCATACCGGTTCGGTCGTACC | Â | Â |
 | 3' reverse | CGCACTAGTCAGGAAGTCCTCTCGCATAC |  |  |
pBBpolB1 all | 5' forward | CGCGAATTCGTCACCTCGGTCCGTGGTAG | Â | Â |
 | 3' reverse | CGCACTAGTGCGTTCGCGGCGACCCAGAGT |  |  |
pBBmcm all | 5' forward | CGCGAATTCGAACAGCATGAACATGCCGA | Â | Â |
 | 3' reverse | CGCACTAGTCGAATACGCCACTGCTAACAA |  |  |
pBBpri2 all | 5' forward | CGCGAATTCGGCGTACTTCCACGTCCAGGG | Â | Â |
 | 3' reverse | CGCACTAGTGCCACGTCCGGGTACGCAGTG |  |  |
pBBrad2 all | 5' forward | CGCGAATTCCCAGCACGAGTCGAGTGGTAA | Â | Â |
 | 3' reverse | CGCACTAGTATCCATGCCTGTGCGTGAGC |  |  |