Figure 1
![Figure 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2156-7-3/MediaObjects/12863_2005_Article_426_Fig1_HTML.jpg)
Rad51 focus formation in BRCA2 -mutated cell lines. a. Cells were treated for 8 hours with MMC (2.4 μg/mL) and immunolabeled for RAD51 (green) and DAPI (blue). MIP101 and RKO are colorectal MSI cancer cells harboring BRCA2 frameshift mutations (see text). BRCA2-/- cells (EUFA423/FA-D1) are derived from a Fanconi anemia patient with complementation group D1/BRCA2 b. Cells were treated with MMC (2.4 μg/mL) or ionizing radiation (12 Gy); after recovery for 8 or 24 hours, cells were stained for RAD51. RAD51 foci were counted and the percentage of cells with > 5 foci was determined. FN1 and VH10 are nonneoplastic BRCA2- wildtype cells.