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Table 1 Cftr mRNA expression in CF/1-CftrTgH(neoim)Hguand CF/3-CftrTgH(neoim)Hgumice

From: Spontaneous rescue from cystic fibrosis in a mouse model

  Log (molecules Cftr mRNA)/μg RNA wild-type/total Cftr mRNA [%]
  Total Cftr mRNAa wild-type Cftr mRNAb  
F8 – F10 CF/1-CftrTgH(neoim)Hgu(n = 10)
Lung 4.7 ± 0.3 3.6 ± 0.3 7.9 ± 2.1
Intestine 5.1 ± 0.2 4.0 ± 0.2 7.1 ± 1.6
F18 CF/1-CftrTgH(neoim)Hgumice (n = 6)
Lung 4.5 ± 0.3 2.9 ± 0.6 2.7 ± 1.9
Intestine 5.3 ± 0.2 4.0 ± 0.2 5.3 ± 1.3
F10 CF/3-CftrTgH(neoim)Hgumouse (n = 1)
Lung 4.5 3.1 4
Intestine 5.0 3.6 4
F18 CF/3-CftrTgH(neoim)Hgumice (n = 6)
Lung 4.9 ± 0.2 < 2 << 0.1
Intestine 5.1 ± 0.2 3.0 ± 0.5 0.8 ± 0.5
Ztm:MF1 mice (positive control, n = 4)
Lung 5.1 ± 0.2 5.1 ± 0.2  
Intestine 5.3 ± 0.1 5.3 ± 0.1  
  1. Reference: log (molecules aldolase mRNA)/μg RNA = 7.26 ± 0.12
  2. acDNA amplified by PCR with mmcftr primer pair A1 – A2;
  3. bcDNA amplified by PCR with mmcftr primer pair B1 – B2 (see Methods section)