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Table 6 PCR Primers used for the MLPH cDNA amplification and/or genomic MLPH mutation analysis

From: Polymorphisms within the canine MLPH gene are associated with dilute coat color in dogs

primer name primer sequence product size TM comments
MLPH cDNA, first round amplification     
Mlph_cDNA_Ex1_F1 CCT TCC TTC CCC TGT AGG AC 513 bp 52°C  
Mlph_cDNA_Ex4_R GGA TCA CCT TGG CAC TCC    
Mlph_cDNA_Ex4_F1 GTG AAG ATC GGC TCG GTA G 686 bp 52°C  
Mlph_cDNA_Ex9_R GGA TGC TGA GAG GTG GTG    
Mlph_cDNA_Ex7_F1 CTT GGA CTT TGA GGC AGA C 985 bp 52°C  
Mlph_cDNA_Ex14_R ACT GAA CTT CCT TCT GAG G    
Mlph_cDNA_Ex10_F AGA GAA GAG GAG ACC CTC 651 bp 52°C  
Mlph_cDNA_Ex16_R GCT GGG TCA TCA CAG GC    
MLPH cDNA, second round amplification     
Mlph_cDNA_Ex1_F2 CTG TAG GAC CGG AGA GAG C 502 bp 52°C  
Mlph_cDNA_Ex4_R GGA TCA CCT TGG CAC TCC    
Mlph_cDNA_Ex4_F2 AGA TCG GCT CGG TAG AGT G 679 bp 52°C  
Mlph_cDNA_Ex9_R GGA TGC TGA GAG GTG GTG    
Mlph_cDNA_Ex7_F2 CTC TGA CGA CTC CAC TTG 967 bp 52°C  
Mlph_cDNA_Ex14_R ACT GAA CTT CCT TCT GAG G    
Mlph_cDNA_Ex10_F AGA GAA GAG GAG ACC CTC 651 bp 52°C same primers as in first round amplification
Mlph_cDNA_Ex16_R GCT GGG TCA TCA CAG GC    
MLPH genomic DNA     
P_Mlph_Ex1_for1 AGT GGC CTC AAG CCC TG 591 bp 56°C  
P_Mlph_Ex1_rev ATG AGC TCC CTG AGA ACC    
P_Mlph_Ex1_for2 CTC CTC CCG AGG CTC CTG 563 bp 56°C  
P_Mlph_Ex1_rev ATG AGC TCC CTG AGA ACC    
Mlph_Ex2_for GTC ACC GAC ATT ATG TCA CAG 598 bp 58°C  
Mlph_Ex2_rev CAG GCT GGA AGG TCA GAT C    
Mlph_Ex3&4_for GAG ACC CAG GAT CGA GTC 725 bp 56°C  
Mlph_Ex3&4_rev CAC TCA CAT TCC AAA GGA TC    
Mlph_Ex5_for AGA AGT GAT GGA GGT CAC TG 471 bp 56°C  
Mlph_Ex5_rev ACC GAA CAG GAA CAG GAG    
Mlph_Ex6_for AG CTT GCC TGG ATG GAT G 582 bp 53°C  
Mlph_Ex6_rev CTG CAG CCT CTG CCA AC    
Mlph_Ex7_for GTC CTC AGC ACT TCT GAG 568 bp 53°C  
Mlph_Ex7_rev GTG AGA AGC TTC TGG ACC    
Mlph_Ex8_for CAG CGG GAT TTC TGA AAG C 483 bp 56°C  
Mlph_Ex8_rev GTG TTG GAC AGT CAG AGT G    
Mlph_Ex9_for CCC GCC TTT GCC TTA AGC 416 bp 56°C  
Mlph_Ex9_rev GCT GCA AGG AGG AGC TTC    
Mlph_Ex10_for CAG AGC CTG GCT CCT GAG 600 bp 56°C  
Mlph_Ex10_rev CAC CTG GGA CAG GGA AGC    
Mlph_Ex11_for AGG CGT CCA GAG GTT GTG 550 bp 55°C  
Mlph_Ex11_rev GCC TGC TTC TGG AGA AGC    
Mlph_Ex12_for CGC CGA CCA AGT CTT TGC 623 bp 55°C  
Mlph_Ex12_rev TGG ACT TGA GGC CGT GTG    
Mlph_Ex13_for CCT GTC TCC CCA GAT TCG 539 bp 55°C  
Mlph_Ex13_rev GGG TTT GCC AAG GCT GAG    
Mlph_Ex14_for CTC CTT TAT GCT CTG GCA C 591 bp 55°C  
Mlph_Ex14_rev TTG TCA CAC GGA GAC ACA G    
Mlph_Ex15_for CTC AGG CAG GCA GAC AAG 461 bp 55°C  
Mlph_Ex15_rev TGC TCT GGG GTC CTA ACG    
P_MLph_Ex16_for GCT TCA GAG CCT GAA ATT CT 469 bp 53°C  
P_Mlph_Ex16_rev GAC AAA AGA TCA GGC TGG AG