Figure 5
From: Analysis of sequence variability in the CART gene in relation to obesity in a Caucasian population
Expression of nuclear proteins in untreated and tunicamycin-treated RIN-1027-B2 cells. GATA-3 and OCT-1 were specifically detected in RIN-untreated cells (lanes 3 and 4). For CHOP, a multiple band pattern was observed (lane 1). As tunicamycin induces endoplasmic stress in cells and consequently enhances CHOP expression, a western blot was performed with tunicamycin treated RIN-1027-B2 cells to determine which band corresponds to CHOP. As pointed by the arrow, the intensity of the band of 27 KDa increased in nuclear extracts from RIN-treated cells, indicating that the amount of CHOP nuclear protein was increased (lane 2 compared to lane 1).