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Table 2 ISSR primers useful for the amplification of the eight populations of A. acutifolius.

From: ISSR markers show differentiation among Italian populations of Asparagus acutifolius L

Primer Name Sequence 5'-3' AT (°C)
3 (CA)8AT 50
4 (CA)8AC 51.7
5 (CA)8GT 51.7
8 (CA)8GAC 54.7
15 GGTC(AC)7 56
16 CGTC(AC)7 56
17 CAGC(AC)7 56
21 CAGC(TC)7 56
23 GAG(TC)8 56
8082 (CT)9G 57
8564 (CAC)7T 58
8565 GT(CAC)7 58
BEC (CA)7YC 54
CHR (CA)7YG 54
DAT (GA)7RC 54
HAD CT(CCT)3CRC 54
MAN CA(CCA)3CRC 54
OH (GAG)7RG 66.7
TE GT(GGT)3GRC 54
W7 (CT)8RG 52.8
W814 (CT)8TG 52
W844 (CT)8RC 52.8
W902 (GT)6AY 39
  1. AT, annealing temperature.