Table 2 ISSR primers useful for the amplification of the eight populations of A. acutifolius.
From: ISSR markers show differentiation among Italian populations of Asparagus acutifolius L
Primer Name | Sequence 5'-3' | AT (°C) |
|---|---|---|
3 | (CA)8AT | 50 |
4 | (CA)8AC | 51.7 |
5 | (CA)8GT | 51.7 |
8 | (CA)8GAC | 54.7 |
15 | GGTC(AC)7 | 56 |
16 | CGTC(AC)7 | 56 |
17 | CAGC(AC)7 | 56 |
21 | CAGC(TC)7 | 56 |
23 | GAG(TC)8 | 56 |
8082 | (CT)9G | 57 |
8564 | (CAC)7T | 58 |
8565 | GT(CAC)7 | 58 |
BEC | (CA)7YC | 54 |
CHR | (CA)7YG | 54 |
DAT | (GA)7RC | 54 |
HAD | CT(CCT)3CRC | 54 |
MAN | CA(CCA)3CRC | 54 |
OH | (GAG)7RG | 66.7 |
TE | GT(GGT)3GRC | 54 |
W7 | (CT)8RG | 52.8 |
W814 | (CT)8TG | 52 |
W844 | (CT)8RC | 52.8 |
W902 | (GT)6AY | 39 |