Table 4 Frequency of auxotrophic mutants among de3-01-CG Ade+ revertants and Ade- survivors. Auxotrophic mutants were detected by replica plating de3-01-CG Ade+ revertants and de3-01-CG Ade- survivors on SDNA+ade and on SDNA+ade + (val, ile, met, arg). The percentage of auxotrophic mutants with respect to the number of tested colonies is reported in parentheses.
No. of replicated colonies | No. of auxotrophic mutants | |
|---|---|---|
de3-01-CG Ade+ revertants | 375 | 41 (10.9%) |
de3-01-CG Ade- survivors | 128 | 10 (7.8%) |