Figure 8

Preferential expression of the maternal allele of Tnfrh1 in fetal and adult tissues from MOLD × BL/6 reciprocal crosses. A, SSCP analysis of RT-PCR products from the BL/6 and MOLD alleles. The mixing experiment validates this method. There is a bias towards greater expression of the maternal allele in several organs, indicated by a relative increase in the MOLD allele intensity in the MxB lanes compared to the BxM lanes. This is superimposed on a non-imprinted baseline of hyper-expression of the BL/6 allele. The allelic bias in placenta reflects expression from maternal cells, but the parent-of-origin dependent bias in other tissues reflects imprinting. Brackets indicate duplicate or triplicate assays using independent aliquots of cDNA. B=BL/6, M=MOLD. B, Direct sequencing of RT-PCR products. Consistent with the SSCP analysis, this shows an expression bias favoring the maternal allele, which is superimposed on a baseline of hyper-expression of the BL/6 allele.