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Table 1 Yeast Strains

From: Isolation and characterization of new Saccharomyces cerevisiae mutants perturbed in nuclear pore complex assembly

Strain Genotype Source
SWY519     MATα ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 Bucci and Wente, 1997
SWY1695     MAT a ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 Bucci and Wente, 1998
SWY2089     MATα lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 this study
SWY2090     MAT a ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 this study
SWY2324     MATα sec13-G176R (npa2-1) lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 backcross of npa2-1 × SWY2090
SWY2325     MATα sec23-S383L (npa1-1) lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 backcross of npa1-1 × SWY2090
SWY2326     MATα sec6-4 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 haploid from SWY2089 × NY17
SWY2327     MATα sec12-4 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 haploid from SWY2090 × CKY39
SWY2328     MATα sec16-2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 haploid from SWY2089 × RSY268
SWY2329     MAT a sar1::URA3 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 p [GAL1-SAR1 LEU2] haploid from SWY2090 × ANY27
SWY2330     MAT a sec31-1 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 haploid from SWY2090 × RSY1004
SWY2331     MATα sec24 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 haploid from SWY2090 × CKY496
SWY2332     MATα sec27(npa3-1) ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 backcross of npa3-1 × SWY202089
SWY2333     MAT a bet3(npa10) ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 backcross of npa10 × SWY2089
SWY2334     MATα np4312 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 original isolate from screen
SWY2335     MATα npa12 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 original isolate from screen
SWY2336     MATα npa67 lys2 ura3-1 his3-11,15 leu2-3,112 can1-100 ade2-1::ADE2:ura3 GFP-nic96:HIS3 nup170-GFP:URA3 original isolate from screen
YCH128     MATα lys2 ura3-1 leu2-3,112 his3-11,15 ade2-1 ade3 C. Hardy
ANY27     MATα sar1::URA3 ura3 trp1 leu2 his3 his4 p [GAL1-SAR1 LEU2] Yamanushi et al., 1996
NY17     MAT a ura3-52 sec6-4 Potenza et al., 1992
CKY39     MATα ura3-52 his4-619 sec12-4 Kurihara et al., 2000
CKY496     MATα ura3-52 leu2-3,112 sec24-1 Kurihara et al., 2000
RSY268     MAT a ura3-52 sec16-2 Kaiser and Schekman, 1990
RSY1004     MATα ura3-52 leu2-3,112 sec31-1 Salama et al., 1997