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Figure 1 | BMC Genetics

Figure 1

From: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing

Figure 1

Promoter sequence of the human EAAT2 (Accession AF510107.1). The primers and the positions of the three SNPs at -200bp (g.-200C > A), -181bp (g.-181A > C) and -168bp (g.-168C > T) are indicated. Numbering is relative to the transcription start site. Primers EAAT2F and EAAT2R were used for standard PCR and Sanger sequencing while EAAT2PyroF-BIO and EAAT2Pyro-R were used to generate biotinylated PCR products and EAAT2PyroSeq1 and EAAT2PyroSeq2 for pyrosequencing (see also Table 1).

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