Table 4 Location of AFLP sequences, list of primers and restriction enzymes for genotyping of SNP markers
From: Population structure of four Thai indigenous chicken breeds
Markers | Chromosome | Forward primer | Reverse primer | Annealing Tem. (°C) | Enzyme |
|---|---|---|---|---|---|
AFLP01 | 2 | 5'-tgtatttccaacctactctac-3' | 5'-gctgtacgtaaggctgcag-3' | 58 | TaqI |
AFLP02 | 3 | 5'-gcacctggaagaatagatag-3' | 5'-gtctttcctggtattcttatg-3' | 56 | EcoRI |
AFLP03 | 4 | 5'-caggaacagcatagaattaaag-3' | 5'-caccagtgaccaaggcaag-3' | 58 | TaqI |
AFLP04 | 15 | 5'-agctgctctcttcagtcag-3' | 5'-agatttcaaagtgtcatgtgc-3' | 58 | TaqI |
AFLP05 | 4 | 5'-tgcaatgcttatctacctgg-3' | 5'-cgtctatctggaaattgctg-3' | 58 | TaqI |
AFLP06 | 11 | 5'-gagaacctgctggtggtg-3' | 5'-tccatgtggcggacgatg-3' | 60 | Hsp92II |
AFLP07 | 4 | 5'-gtttcagcaaggcagagttc-3' | 5'-gaccaaacaacgtcacgttc-3' | 58 | TaqI |
AFLP08 | 6 | 5'-tttgtttcagcaaggcagag-3' | 5'-gaccaaacaacgtcacgttc-3' | 58 | TaqI |
AFLP09 | 1 | 5'-aaccatgcagccagagaatg-3' | 5'-gcagccattcatacatggtc-3' | 58 | EcoRI |
AFLP10 | 14 | 5'-gctctgatcaaagacattcc-3' | 5'-gatcatcttcggctacagag-3' | 58 | AluI |
AFLP11 | 11 | 5'-gtgcagctggggttggg-3' | 5'-ggaaccggtgcttgcattg-3' | 58 | BsuRI |
AFLP12 | 1 | 5'-tgaccatgctttctccatag-3' | 5'-tttggaaatcaattttcagctc-3' | 58 | TaqI |
AFLP13 | 1 | 5'-gcagctgcgtataaacacag-3' | 5'-caggactgcagggataaatg-3' | 58 | TaqI |
AFLP14 | 10 | 5'-gcttcagcaggcagatttc-3' | 5'-ctttacgtggcccaccttc-3' | 58 | BsuRI |
AFLP15 | 2 | 5'-aaactcttttcccttggctg-3' | 5'-gtgcaagacggactgtattg-3' | 58 | EcoRI |
AFLP16 | 8 | 5'-actcgcaggagaacttcttag-3' | 5'-gcagtcctgtgacttatttg-3' | 58 | EcoRI |
AFLP17 | 1 | 5'-gaaatcgttgccaaaagttgc-3' | 5'-gttcacgcagctcggatg-3' | 58 | MspI |
AFLP18 | 7 | 5'-ggtttgatttctgggatctc-3' | 5'-gccttaggtaacattccttc-3' | 58 | EcoRI |
AFLP19 | 2 | 5'-cctcctactgattctgtaatg-3' | 5'-ttttctgctcatctgtactgg-3' | 56 | Hin6I |
AFLP20 | 4 | 5'-aggatcacaaataaccaacga-3' | 5'-gactacagtgagaagctctg-3' | 60 | MboI |
AFLP21 | 10 | 5'-gtctgcacacctggtgtc-3' | 5'-caggttcacacggagatc-3' | 58 | MspI |
AFLP22 | 1 | 5'-ggaggttcgtgagaagctg-3' | 5'-tgtacaacagcagcaagcaaa-3' | 60 | Hsp92II |
AFLP23 | 8 | 5'-ctttctccttctccccaagt-3' | 5'-cttggatagggtctgcaga-3' | 58 | TaqI |
AFLP24 | 1 | 5'-gcagtgcacctggattttag-3' | 5'-attatcccttccctcagctg-3' | 60 | TaqI |
AFLP25 | 17 | 5'-ctgctagcaggtaatgagat-3' | 5'-tggcagaaagattccgtcaa-3' | 55 | TaqI |
AFLP26 | 2 | 5'-tctctgctagtgtgtgtgga-3' | 5'-caccaggactgaagaacaga-3' | 60 | EcoRI |
AFLP27 | 3 | 5'-ctgagaatagccaggacaca-3' | 5'-gtgttgggaatttaggaaac-3' | 58 | TaqI |
AFLP28 | 11 | 5'-gtgagggcaaccagagcca-3' | 5'-tgaagattcctgttcttgag-3' | 58 | Hsp92II |
AFLP29 | 9 | 5'-ctgctgcggaccgaaatatc-3' | 5'-gatagttccgaacagtttgc-3' | 58 | BsuRI |
AFLP30 | 13 | 5'-cagatgatcacagtaacctg-3' | 5'-gtggaacttgtaagtacgc-3' | 58 | TaqI |