Table 4 Virtual restriction mapping of 3.56 kb unit of DYZ1 array from MZT1 (both blood and germline DNA) using Restriction Mapper software
From: DYZ1 arrays show sequence variation between the monozygotic males
Sr. No. | Restriction Site | Sequence | Overhang | Restriction Enzyme Site Frequency | |||
|---|---|---|---|---|---|---|---|
MZT1a (Blood) | MZT1a (Germline) | MZT1b (Blood) | MZT1b (Germline) | ||||
1 | Cac8I | GCNNGC | 5’ | 1 | 1 | 1 | 1 |
2 | BseYI | CCCAGC | 5’ | 1 | 1 | 1 | 1 |
3 | Bsp1407I | TGTACA | 5’ | 1 | 1 | 1 | 1 |
4 | BspHI | TCATGA | 3’ | 1 | 1 | 1 | 1 |
5 | ClaI | ATCGAT | 3’ | 1 | 1 | 1 | 1 |
6 | DdeI | CTNAG | blunt | 1 | 1 | 1 | 1 |
7 | EcoRI | GAATTC | 5’ | 1 | 1 | 1 | 1 |
8 | ScrFI | CCNGG | 3’ | 1 | 1 | 1 | 1 |
9 | VspI | ATTAAT | blunt | 1 | 2 | 1 | 2 |
10 | BseMII | CTCAG | 5’ | 1 | 1 | 1 | 1 |
11 | BsgI | GTGCAG | 3’ | 1 | 1 | 1 | 1 |
12 | BtsI | GCAGTG | 3’ | 1 | 1 | 1 | 1 |
13 | Eco57I | CTGAAG | 3’ | 1 | 1 | 1 | 1 |
14 | GsuI | CTGGAG | 3’ | 1 | 1 | 1 | 1 |
15 | Hpy188I | TCNGA | 3’ | 1 | 1 | 1 | 1 |
16 | MnlI | CCTC | 3’ | 1 | 1 | 1 | 1 |
17 | PflMI | CCANNNNNTGG | 3’ | 1 | 1 | 1 | 1 |
18 | SduI | GDGCHC | blunt | 1 | 1 | 1 | 1 |
19 | AcyI | GRCGYC | 5’ | 0 | 0 | 0 | 1 |
20 | HgaI | GACGC | 5’ | 0 | 0 | 0 | 1 |
21 | Hpy99I | CGWCG | 3’ | 0 | 0 | 0 | 1 |
22 | HaeIV | GAYNNNNNRTC | 3’ | 0 | 0 | 0 | 2 |
23 | Hin4I | GAYNNNNNVTC | 3’ | 0 | 0 | 0 | 2 |
24 | DpnI | GATC | 5’ | 2 | 2 | 2 | 3 |
25 | FokI | GGATG | 3’ | 2 | 2 | 2 | 3 |
26 | MboI | GATC | 5’ | 2 | 2 | 2 | 3 |
27 | ArsI | GACNNNNNNTTYG | 5’ | 2 | 2 | 2 | 2 |
28 | BdaI | TGANNNNNNTCA | 5’ | 2 | 2 | 2 | 2 |
29 | CspCI | CAANNNNNGTGG | 5’ | 2 | 0 | 0 | 0 |
30 | Eco57MI | CTGRAG | 5’ | 2 | 2 | 2 | 2 |
31 | SetI | ASST | 5’ | 2 | 3 | 2 | 2 |
32 | TspRI | CASTG | 5’ | 2 | 2 | 2 | 3 |
33 | ApoI | RAATTY | 3’ | 3 | 3 | 3 | 3 |
34 | AsuII | TTCGAA | 3’ | 3 | 4 | 3 | 3 |
35 | Eco31I | GGTCTC | blunt | 3 | 3 | 3 | 3 |
36 | TatI | WGTACW | 5’ | 3 | 3 | 3 | 3 |
37 | MboII | GAAGA | 3’ | 3 | 3 | 4 | 4 |
38 | CviJI | RGCY | blunt | 4 | 4 | 4 | 4 |
39 | MslI | CAYNNNNRTG | 5’ | 4 | 4 | 4 | 4 |
40 | BccI | CCATC | 3’ | 4 | 4 | 4 | 4 |
41 | MaeI | CTAG | 3’ | 4 | 4 | 4 | 3 |
42 | SfaNI | GCATC | 3’ | 4 | 4 | 4 | 5 |
43 | AlfI | GCANNNNNNTGC | 3’ | 4 | 4 | 4 | 4 |
44 | TstI | CACNNNNNNTCC | 3’ | 4 | 4 | 4 | 4 |
45 | RsaI | GTAC | 3’ | 5 | 5 | 5 | 5 |
46 | BsmAI | GTCTC | 3’ | 5 | 5 | 5 | 5 |
47 | BstXI | CCANNNNNNTGG | blunt | 5 | 5 | 4 | 5 |
48 | XmnI | GAANNNNTTC | 5’ | 6 | 6 | 6 | 6 |
49 | MseI | TTAA | 3’ | 6 | 6 | 6 | 6 |
50 | NlaIII | CATG | 5’ | 7 | 7 | 7 | 7 |
51 | BcgI | CGANNNNNNTGC | 5’ | 14 | 12 | 14 | 14 |
52 | BsrDI | GCAATG | 5’ | 15 | 14 | 14 | 15 |
53 | BsrI | ACTGG | 5’ | 19 | 19 | 18 | 19 |
54 | TspGWI | ACGGA | 5’ | 19 | 19 | 19 | 19 |
55 | BsmI | GAATGC | 5’ | 21 | 20 | 20 | 20 |
56 | AgsI | TTSAA | 5’ | 22 | 23 | 22 | 23 |
57 | FaiI | YATR | 3’ | 23 | 23 | 23 | 24 |
58 | PleI | GAGTC | 3’ | 23 | 24 | 23 | 24 |
59 | TspDTI | ATGAA | blunt | 25 | 24 | 25 | 24 |
60 | TspEI | AATT | 5’ | 34 | 35 | 32 | 35 |
61 | TfiI | GAWTC | 3’ | 53 | 52 | 53 | 51 |
62 | TaqI | TCGA | blunt | 66 | 65 | 67 | 66 |
63 | HinfI | GANTC | 5’ | 76 | 76 | 76 | 75 |