Table 4 Primer sequences used in qPCR for SCNN1 gene family members and internal control gene β-actin
Primer name | Sequence (5’→3’) | Tm(°C) | Product size (bp) | Position |
|---|---|---|---|---|
SCNN1a-F | TCATGTTCAGCGCCATCCT | 60 | 175 | Exon3+4 628-802 |
SCNN1a-R | TTCCCGCACTGCATCCA | 60 | Â | |
SCNN1b-F | GAAGTTCCCAGCAGTCACAGTCT | 60 | 168 | Exon2+3 277-444 |
SCNN1b-R | GTGGCGTTGCTGTTGTTCAG | 60 | Â | |
SCNN1d-F | CACCATCCACGGCACCAT | 60 | 183 | Exon1 90-272 |
SCNN1d-R | AACATCTTGGGCTCCGAATG | 60 | Â | |
SCNN1g-F | TGGGACAAAGGACAGAAAATC | 60 | 141 | Exon10+12 1450-1590 |
SCNN1g-R | GCCGAAGTTGGACAGAAGGA | 60 | Â | |
β-actin-F | TATGTGCAAGGCCGGTTTC | 60 | 110 | Exon1+2 113-222 |
β-actin-R | TGTCTTTCTGGCCCATACCAA | 60 |  |