Figure 7
From: Carriers of a novel frame-shift insertion in WNT16a possess elevatedpancreatic expression of TCF7L2
Reporter constructs assembled with the mutated form of the Wnt16a5′ UTR are more efficiently translated than the wild-typeform. The wild-type and mutant Wnt16a 5′ UTR sequenceswere inserted adjacent to a luciferase cDNA, and the resulting plasmidswere used to transfect HEK-293 cells. 48 hours after transfection,cells were lysed, and firefly luciferase (FFL) and Renillaluciferase (RL) levels were measured. Our reporter constructs using thewild-type and the mutant (insertion) sequence of Wnt16a showedsignificantly increased (70%, p<0.0001) levels of luciferase proteinin the constructs carrying the mutant sequence.