A missense mutation in growth differentiation factor 9 (GDF9) is strongly associated with litter size in sheep

Table 3 Primers used for amplification, sequencing and genotyping of the ovine GDF9 gene

Name	Direction	Position	Sequence5’-3’
7916	Forward	1744 - 1763^a	ATGGGGAAATGTGTTCCTTG
7917	Reverse	2187 - 2206^a	CCACCCATTAACCAATCTGC
7918	Forward	3205 - 3224^a	GGGGAGAAAAGGGACAGAAG
7919	Reverse	4283 - 4302^a	GCCAGGACACTCATGGTTTT
7979	Forward	3863 - 3882^a	AGGAGAGTGCCAGCTCTGAA
7980	Reverse	4443 - 4462^a	CATGAGGAAGGCAGCTGTTA
GDF9F	Forward	3983 - 4002^a	GCTTTAGTCAGCTGAAGTGG
GDF9R	Reverse	4039 - 4058^a	CAGTCCCCTTTACAGTATCG
GDF9E	Extension	3996 - 4014^a	GAAGTGGGACAACTGGATT

^aPositions are numbered according to the genomic sheep GDF9 sequence (Accession number: AF078545.2).

ISSN: 2730-6844