Schematic representation and primer placement of the predicted structure of WC1 Domain 1. Secondary structure motifs are indicated above (beta-strand arrows (β1-β6) and an alpha-helix arrow). The RVEVLxxxxW bacterial binding motif is circled. The two regions (VR1 and VR2) that contain most of the sequence diversity between WC1 SRCR domain 1s are boxed. Cysteines are indicated in black . The common forward and reverse primers were designed based on the conserved region and denoted by a single asterisk (*), while the specific forward primers for each WC1 gene are denoted by double asterisks (**).