Figure 5

ssl1-1 mutation affects nuclear accumulation of poly(A)⁺RNA in mex67-5 mutant cells. Single mex67-5 and double mex67-5 ssl1-1 [either transformed with the empty vector (vector) or SSL1 in a CEN plasmid (pSSL1)] mutant cells were grown to mid-log phase at 23 °C and incubated for 1 hour at 37 °C. In all the cases, cells were fixed and in situ hybridization was performed using a digoxigenin-conjugated oligo(dT) probe, followed by incubation with a FITC-conjugated anti-digoxigenin antibody [poly(A)]. DNA was visualized by DAPI staining.