Reaction conditions | Primers | Standards | Telomere | Reference | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Assay | Platform | Type | Master mix | Duration | Tel | Ref | Batches | Dilutions | LDR | Cq | NTC | Cq | NTC |
I | ABI | Singleplex | Commercial | 21 | CW2006 | BA 1998 | 7 | 5 | 4.5-72.0 | 17.6 | 34.9 | 33.5 | 40.0 |
II | RG | Multiplex | RC 2009 | 6 | RC 2009 | RC 2009 | 4 | 4 | 2.2-60.0 | 15.7 | 19.4 | 39.9 | 27.9 |
III | RG | Singleplex | RC 2009 | 5 | RC 2009 | RC 2009 | 4 | 4 | 2.2-60.0 | 14.1 | 15.5 | 37.4 | 36.2 |
IV | RG | Multiplex | Commercial | 6 | RC 2009 | RC 2009 | 4 | 5 | 4.5-72.0 | 13.9 | 27.2 | 34.3 | 31.1 |