Table 3 MSAP primer sequences used in this work
From: Methylation changes associated with early maturation stages in the Atlantic salmon
Oligo name | Function | Sequence |
|---|---|---|
Ad.Hpa II/Msp I Rv | Adaptor | GACGATGAGTCTAGAA |
Ad.Hpa II/Msp I Fw | Adaptor | CGTTCTAGACTCATC |
Ad.Eco RI Rv | Adaptor | AATTGGTACGCAGTCTAC |
Ad.Eco RI Fw | Adaptor | CTCGTAGACTGCGTACC |
Pre. Eco RI | Preselective primer | GACTGCGTACCAATTCA |
Pre. Hpa II/Msp I | Preselective primer | GATGAGTCTAGAACGGT |
Eco RI + ACT | Selective primer | GACTGCGTACCAATTCACT |
Eco RI + AAG | Selective primer | GACTGCGTACCAATTCAAG |
Hpa II + TAC | Selective primer | GATGAGTCTAGAACGGTAC |
Hpa II + TC | Selective primer | GATGAGTCTAGAACGGTC |