Table 3 Primers utilized for POMC genotyping and characterization of variants.
POMC target | Primer | Sequence (5' to 3') |
|---|---|---|
POMC c.288C>T | Forward | GATGAGCAGCCGCTGACT |
| Â | Reverse | GTCAGCTCCCTCTTGAATTCGAG |
| Â | Anchor | GCTTCGGCCGTCGGAATGGT |
| Â | Sensor | GCAGCAGCAGCGGAGTTG |
12 bp deletion | Forward | CCGTCGGAATGGTAGCA |
| Â | Reverse | CGTTGGGGTACACCTTC |
cDNA | Forward | GCGACGGAAGAGAACGAAGGA |
| Â | Reverse | TGATGGCGTTTTTGAACAGCGTGAC |