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Table 1 Characteristics of 25 newly developed sugar beet microsatellite markers.

From: Characterisation of sugar beet (Beta vulgaris L. ssp. vulgaris) varieties using microsatellite markers

Micro-satellite marker1

EMBL Accession number

Forward primer (5'-3')

Reverse primer2 (5'-3')

Repeat motif3

Annealing Temp

Predicted size

Quality4

Bvv01

[EMBL:AM410749]

CCATATGGAGGGGTAGAGCA

(GGA)4-1(GGT)7

55

105

1

  

GTTTGCACCATAGGCACCACCACTTG

    

Bvv10

[EMBL:AM410750]

CTTTGAGAATTGAGATACTATG

(CA)56-3

52

212

1

  

GTTTGTCTGGACGCAAGCACAC

    

Bvv15 5

[EMBL:AM410751]

TGCTGACCTTGCAGTTAATAAGTT

(CAA)34-7

54

298

1

  

GTTTCATGTGATGGCTTGCTTTCTAA

    

Bvv17

[EMBL:AM410752]

CGACGCCTTTTTGAAGGAATAGGAT

(GAT)12-3

57

128

1

  

GTTTCACCCCTGGGTCCTGATCTACAAC

    

Bvv186

[EMBL:AM410753]

CACCATAACCGCCCCCACCATAAT

(GCC)3(ACC)3

60

208

1

  

GTTTCTTGGCCGTAGGGTAAGGGTCAACTA

    

Bvv21

[EMBL:AM410754]

TTGGAGTCGAAGTAGTAGTGTTAT

(GGC)13-5

53

250

1

  

GTTTATTCAGGGGTGGTGTTTG

    

Bvv22

[EMBL:AM410755]

CTATGCATCGCCCAATAATTACTTAA

(CCG)5(CCA)5

52

210

1

  

GTTTATATAACACTGCTTATTTAATGTCC

    

Bvv23

[EMBL:AM410756]

TCAACCCAGGACTATCACG

(GA)16

50

109

1

  

GTTTACTGACAAAGCAAATGACCTACTA

    

Bvv257

[EMBL:AM410757]

GAAACCACATAAAAACCCCTCTTA

(TCA)10

51

121

2

  

GTTTCAAGTAGTCCCGTTAACATCTGA

    

Bvv27

[EMBL:AM410758]

GGGTTCATCATCATCCTTATCATT

(TCA)13-3, (TCA)35-10

54

310

1

  

GTTTACGCTCCTCCATCATCAGACCA

    

Bvv30

[EMBL:AM410759]

TGTGCCCAAAATCCTGAA

(GA)31

51

183

1

  

GTTTAATTGGCTGGGTAAAAGAGA

    

Bvv31

[EMBL:AM410760]

AGAAGCCTTTAAAATCCAACT

(CA)14, (GTAT)69

49

461

2

  

GTTTACAGCGTCTCACCATAAGT

    

Bvv32

[EMBL:AM410760]

AGAAGCCTTTAAAATCCAACT

(CA)14

50

142

2

  

GTTTACATATGGAACTTTAATGAACAAGTGATAT

    

Bvv37

[EMBL:AM410761]

TGGACGCCATATTAGAAGAT

(GT)27

50

216

1

  

GTTTATACAAATGAATATGAGAATACTG

    

Bvv43

[EMBL:AM410762]

TGACACTCTTCTTTGCAACACATAA

(GT)96-18

54

257

2

  

GTTTGTAAATGTTGCAAAATATTGGTAT

    

Bvv45

[EMBL:AM410763]

GTATAGCAAAAGTCATTTTGTTTGTGT

(GT)14-1, (CGCA)8

55

230

1

  

GTTTCTCGGCCTTCCCTTTCTAATGTCTAG

    

Bvv48

[EMBL:AM410764]

GGCTTCCCTAGACAACC

(GT)24

50

209

1

  

GTTTATAGGCAAATGAATGAGG

    

Bvv51

[EMBL:AM410765]

AGCAAAACTTATCTCAAATCTGG

(TG)9(AG)32-1

51

272

1

  

GTTTGTCTACCGTGGCTGTGC

    

Bvv53

[EMBL:AM410766]

CATGTCGAGGAGTGAGTTCAGGAA

(GT)17, (GA)35

53

226

1

  

GTTTCAACTATAGGTGCATCTTTTAC

    

Bvv54

[EMBL:AM410767]

ATCTGCATGCCGTCACTC

(TC)12(AC)12

52

279

1

  

GTTTCACTGTACCTTCGAATGTTAG

    

Bvv57

[EMBL:AM410768]

CATTACCATGGGAACGAA

(GT)25

50

232

1

  

GTTTAAGGGATACAATGTTAGTTATGAA

    

Bvv60

[EMBL:AM410769]

AAGAATGCTTCAACTTTTTCATGG

(CAT)7, (CAT)7, (CAT)11-1

52

256

1

  

GTTTAGGGTCGGATATAAGAGGGAGTGG

    

Bvv61

[EMBL:AM410770]

ATGGGAGAATATTGGTGACA

(GAA)22

49

200

1

  

GTTTGCCACAAATCATCTCTACTAA

    

Bvv62

[EMBL:AM410771]

ATGGCAATGCGCAGAATAACC

(CAG)11-2

54

155

1

  

GTTTGCTGAGGAGGCTGCATTTGTT

    

Bvv64

[EMBL:AM410772]

TTTTTGGGAGTTTCATCACTACTTT

(CT)18-1(CA)19

51

207

2

  

GTTTCATATAAGGGGAGTCTTCTCACAA

    
  1. 1 In bold the 12 markers that have been used to genotype 40 cultivars (1200 plants) (see Table 2). They were amplified separately but combined before analysis on an ABI sequencer, as follows: multiplex 1 consisted of markers Bvv15, Bvv30, and Bvv64; multiplex 2 of Bvv17, Bvv43, and Bvv61; multiplex 3 of Bvv 51, Bvv 53, and Bvv60; multiplex 4 of Bvv 21, Bvv23, and Bvv32
  2. 2 GTTT is a pigtail [24]
  3. 3 the number after the minus sign is the number of imperfect repeats. For instance, (CA)56-3 means that the microsatellite repeat covers of length of 56 (CA) repeat units, but of these 3 are not (CA).
  4. 4 according to Smulders et al. [25]
  5. 5 The sequence of the forward primer of Bvv15 was found in cDNA clone EO12340
  6. 6 The sequence of the cloned Bvv18 fragment was found in cDNA clone EG551697
  7. 7 The sequence of the cloned Bvv25 fragment was found in BAC clone ED032383
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