Table 2 Dual color FISH used to distinguish the chromosomes pairs of the Regions 2, 3 and 4.

Region 2

S2B × S2C

1 (color of S2C); 2 (color of S2B); 16 (two color)

R2 × S2A

3 (color of R2); 1, 2, 16 (two color)

R2 × S2B

2, 16 (two color); 1, 3 (color of R2) - define pair 3 for exclusion of pair 1

Region 3

S3B × S3C

7 (two color); 19 (color of S3B)

S3A × S3D

18 (color of S3D); 5, 6, 7, 17 (two color)

S3A × S3B

7 (two color); 19 (color of S3B); 5, 6, 17 (color of S3A)

R3 × S3D

5, 6, 7, 17, 18 (two color); 4, 8, 19 (color of R3) - define pairs 4,8 for exclusion of pair 19

Region 4

S4A × S4B

14 (color of S4B); 12, 13, 15 (two color)

R4 × S4C

9, 14 (color of R4); 10, 11, 12, 13, 15, 21 (two color) - define pair 9 for exclusion of pair 14

S4B × S4D

21 (color of S4D); 12, 13, 14, 15 (two color)

S4C × S4D

14 (color of S4D); 10, 11 (color of S4C), 12, 13, 15, 21 (two color)