Analysis of ROCK1/Little Rock nucleotide differences. (A) Chromatograms from amplified genomic DNA that includes both ROCK1 and Little ROCK. Gene specific exon amplification was not possible due to high sequence identity. The location of variants identified in dbSNP is shown. Overlapping chromatogram peaks with similar sizes indicates locations of sequence variability. (B) Chromatograms from amplified cDNA. The use of a primer specific for the Little ROCK-specific exon and a primer specific to ROCK1 ensured the specificity of each amplicon. No ambiguous bases were found in the ROCK1 cDNA while rs1045142 and rs2663698 were heterozygous in the Little ROCK cDNA amplicon. (C) The location of fixed sequence differences created by ROCK1 and Little ROCK sequence differences (rs2847092 and rs1045144) and Little ROCK Haplotype AC and Haplotype GT created by linkage disequilibrium between polymorphisms rs1045142 and rs2663698 are shown. Sequence findings represent the results from 90 unique Caucasian DNA samples and from cultured VSMC cDNA analysis.