Neighbor joining tree of 11 NBS genes in the REN1 interval (bold) and their most similar paralogues in the grape genome, which are 3 NBS genes in the interval chr13:11828966..12013448 (italics) and 11 NBS genes in the interval chr13:21506564..21871189 (underlined). The NJ coefficient is based on amino acid alignments. Bootstrapping was performed with 1000 replicates and the percentage of replicates supporting that branch is given above the branch (if >70). The estimates of average evolutionary divergence between NBS-LRR sequences within groups are indicated in parentheses above the corresponding branch. The number of amino acid substitutions per site from averaging over all sequence pairs within each group was calculated using the Poisson correction method in MEGA. All positions containing alignment gaps or missing data were eliminated only in pairwise sequence comparisons (Pairwise deletion option).