Table 5 Primer sequences and PCR conditions
localization | primer | annealing Tm [°C] | |
|---|---|---|---|
1 | promoter | GAATGCCTCTTCCTGAATGG CCTCCTTGCCTTTGATATTGA | 60 |
2 | promoter | GGCAAGGAGGTTAACATCTGA GAGACTCCCCTGAATCACCA | 60 |
3 | promoter | GCAGCACAGTTTCCTCCAG GCTGCTTGCCTATCCACTTC | 60 |
4 | promoter, exon 1, intron 1 | GGATTAATGGGAAAAGTTTTGG AGCAACTAACGACCGTGGAC | 59 |
5 | intron 1, exon 2 | TCCAGGATTGAGAAAAATCTGC CAAGAATCCTAAACCTGGGATG | 60 |
6 | exon 3, intron 3 | TCACCCTCTCATCCTCTACACC GCTGATTAGCGATAGAGTGACC | 60 |
7 | exon 4, intron4 | CTGCCCCTGCTGTGTCTG AGGAAGAACCTACAAGCACCAC | 65 |
8 | intron 4 | GCTTCCACTACGGAGTCCAC GATGAGGGAGGGTGAGAAAAG | 59 |
9 | exon 5 | AACCATCTTTCTCCCTTCTTCG GCACTCCCTTCTGTCTCTGG | 60 |
10 | intron 5 | GCTGGGCATGTCTCACAAC CAAAGCGAATGGCTGCATAG | 59 |
11 | exon 6, intron 6, exon 7 | CTACAGCGCCTACCTGAAAAAC GAGAGCCAGGTCACTATCATCG | 60 |
12 | intron 5, exon 6, intron 6, exon 7 | GCATCTCTGGGGAGTTCCTA TCGTTGAGGAAGAGGTGGTC | 60 |
13 | exon7, intron 7 | TCTCTGTCTTTGCTCGTGTACC CCAGGAGGGCTGAGTGTG | 67 |
14 | intron 7 | TAGTGACCTGGCTCTCTTCATC ATGGCCTCCACCTGTGAC | 59 |
15 | exon 8 | CCAAGGTCCCCTGTCCTC GAGAGGAGGCAGGGCTATAAG | 60 |