|
Chromosome containing ura3::HIS3
|
Galactose exposure prior to selection
|
Frequency of Ura+
|
|---|
|
III
|
-
|
3.4 ± 3.2 × 10-4
|
|
IV
a
|
-
|
1.0 ± 0.0 × 10-4
|
|
VI
b
|
-
|
9.7 ± 1.8 × 10-5
|
|
VI
b
|
+
|
1.3 ± 0.6 × 10-4
|
- Haploid cells were grown overnight in YPD rich medium. 106 cells were plated onto selective medium lacking uracil. Colonies were counted after 3 days. The mean frequency and standard deviation obtained from at least 4 independent cultures are shown.
- aOnly 2 independent cultures were tested.
- bCultures were grown in supplemented minimal medium containing raffinose. Where indicated, galactose was added to 1.5% and culture was grown for 1.3 doublings prior to plating onto selective medium. Diluted cultures were also plated to YPD to score viable cell density. Ura+ frequency was computed per viable cell plated. A paired t-test indicates that the frequencies were not different (2-tailed, P > 0.1).
