A strategy for constructing aneuploid yeast strains by transient nondisjunction of a target chromosome

BMC Genomic Data

Table 1 Frequency of Ura⁺ cells in haploid strains containing ura3::HIS3 integrated next to a centromere

Chromosome containing ura3::HIS3	Galactose exposure prior to selection	Frequency of Ura⁺
III	-	3.4 ± 3.2 × 10^-4
IV ^a	-	1.0 ± 0.0 × 10^-4
VI ^b	-	9.7 ± 1.8 × 10^-5
VI ^b	+	1.3 ± 0.6 × 10^-4

Haploid cells were grown overnight in YPD rich medium. 10⁶ cells were plated onto selective medium lacking uracil. Colonies were counted after 3 days. The mean frequency and standard deviation obtained from at least 4 independent cultures are shown.
^aOnly 2 independent cultures were tested.
^bCultures were grown in supplemented minimal medium containing raffinose. Where indicated, galactose was added to 1.5% and culture was grown for 1.3 doublings prior to plating onto selective medium. Diluted cultures were also plated to YPD to score viable cell density. Ura⁺ frequency was computed per viable cell plated. A paired t-test indicates that the frequencies were not different (2-tailed, P > 0.1).

ISSN: 2730-6844