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Table 1 Frequency of Ura+ cells in haploid strains containing ura3::HIS3 integrated next to a centromere

From: A strategy for constructing aneuploid yeast strains by transient nondisjunction of a target chromosome

Chromosome containing ura3::HIS3 Galactose exposure prior to selection Frequency of Ura+
III - 3.4 ± 3.2 × 10-4
IV a - 1.0 ± 0.0 × 10-4
VI b - 9.7 ± 1.8 × 10-5
VI b + 1.3 ± 0.6 × 10-4
  1. Haploid cells were grown overnight in YPD rich medium. 106 cells were plated onto selective medium lacking uracil. Colonies were counted after 3 days. The mean frequency and standard deviation obtained from at least 4 independent cultures are shown.
  2. aOnly 2 independent cultures were tested.
  3. bCultures were grown in supplemented minimal medium containing raffinose. Where indicated, galactose was added to 1.5% and culture was grown for 1.3 doublings prior to plating onto selective medium. Diluted cultures were also plated to YPD to score viable cell density. Ura+ frequency was computed per viable cell plated. A paired t-test indicates that the frequencies were not different (2-tailed, P > 0.1).