Table 4 Association of different genotypes of SNPs in PRKG2 gene with number of thoracolumbar vertebrae and carcass traits in Dezhou donkey. Values with different letters (a > b; A > B) within the same row denote significance levels of P < 0.05 and P < 0.01, respectively

(cm)

(cm)

(cm)

(kg)

(cm)

(cm)

g.162140112A > G

AA/30

135.60 ± 4.28

133.05 ± 5.01

146.25 ± 4.93

154.85 ± 17.05

5.23 ± 0.43ab

24.43 ± 2.82ab

17.83 ± 0.38

73.08 ± 3.56

23.07 ± 0.25

AG/162

134.87 ± 5.24

132.68 ± 5.84

145.08 ± 5.17

152.27 ± 14.91

5.14 ± 0.34b

23.78 ± 1.80b

17.91 ± 0.33

72.93 ± 3.56

23.04 ± 0.32

GG/213

134.72 ± 5.02

132.39 ± 6.58

144.60 ± 5.20

150.42 ± 22.25

5.26 ± 0.44a

24.31 ± 2.24a

17.83 ± 0.42

72.73 ± 3.68

23.09 ± 0.37

P-value

0.667

0.816

0.227

0.401

0.011

0.042

0.117

0.809

0.442

g.162149155G > C

GG/61

134.07 ± 44.52ab

132.10 ± 6.30

144.93 ± 4.74

151.92 ± 15.18

5.21 ± 0.41

24.28 ± 2.34

17.85 ± 0.36

72.65 ± 3.42

23.07 ± 0.31

GC/183

135.62 ± 5.05a

133.03 ± 6.12

145.53 ± 5.14

152.72 ± 19.84

5.17 ± 0.38

23.95 ± 2.11

17.89 ± 0.35

73.07 ± 3.55

23.06 ± 0.33

CC/162

134.26 ± 5.13b

132.16 ± 6.20

144.24 ± 5.30

149.94 ± 19.89

5.25 ± 0.44

24.24 ± 2.10

17.83 ± 0.43

72.62 ± 3.77

23.08 ± 0.37

P-value

0.018

0.359

0.071

0.399

0.208

0.369

0.373

0.466

0.861

g.162149571C > T

CC/62

134.02 ± 4.87

131.94 ± 6.11

145.11 ± 4.91

152.31 ± 15.61

5.23 ± 0.42

24.34 ± 2.46

17.84 ± 0.37

72.58 ± 3.18

23.06 ± 0.31

CT/191

135.52 ± 5.19

133.34 ± 6.37

145.27 ± 5.41

152.86 ± 20.29

5.16 ± 0.37

23.97 ± 2.04

17.89 ± 0.35

73.17 ± 3.81

23.05 ± 0.32

TT/153

134.33 ± 4.85

131.80 ± 5.88

144.41 ± 4.96

149.44 ± 19.15

5.27 ± 0.44

24.20 ± 2.12

17.82 ± 0.43

72.50 ± 3.52

23.09 ± 0.39

P-value

0.035

0.051

0.295

0.244

0.056

0.402

0.257

0.201

0.575

g.162153251G > A

GG/262

134.66 ± 4.92

132.41 ± 6.12

144.80 ± 5.31

151.98 ± 18.75A

5.21 ± 0.41

24.09 ± 2.13

17.85 ± 0.38

72.73 ± 3.52

23.06 ± 0.32

GA/55

135.01 ± 4.25

132.27 ± 5.68

145.23 ± 4.08

152.63 ± 13.26A

5.20 ± 0.40

24.09 ± 1.97

17.85 ± 0.41

72.85 ± 3.43

23.05 ± 0.30

AA/27

133.26 ± 6.22

130.72 ± 6.55

142.61 ± 5.59

138.59 ± 31.64B

5.30 ± 0.47

23.98 ± 2.53

17.81 ± 0.48

71.48 ± 3.46

23.11 ± 0.58

P-value

0.300

0.392

0.079

0.002

0.559

0.970

0.893

0.190

0.754

g.162156524C > T

CC/258

134.70 ± 4.96

132.35 ± 6.15

144.64 ± 5.20

151.98 ± 15.99A

5.20 ± 0.40

24.08 ± 2.07

17.85 ± 0.38

72.75 ± 3.59

23.05 ± 0.33

CT/99

135.37 ± 5.10

132.87 ± 6.56

145.47 ± 4.92

152.67 ± 22.27A

5.21 ± 0.41

24.19 ± 2.23

17.87 ± 0.40

73.27 ± 3.83

23.08 ± 0.34

TT/24

133.21 ± 5.90

131.02 ± 5.13

144.02 ± 5.31

139.00 ± 31.88B

5.29 ± 0.46

23.98 ± 2.59

17.83 ± 0.48

71.69 ± 3.04

23.13 ± 0.54

P-value

0.155

0.414

0.288

0.005

0.585

0.877

0.902

0.140

0.556

g.162158453C > T

CC/245

134.72 ± 4.79ab

132.41 ± 6.08

144.70 ± 5.13ab

152.03 ± 15.69a

5.22 ± 0.41

24.10 ± 2.08

17.85 ± 0.38

72.75 ± 3.58

23.07 ± 0.34

CT/139

135.43 ± 5.16a

133.14 ± 6.37

145.69 ± 5.02a

152.12 ± 24.65a

5.19 ± 0.40

24.16 ± 2.20

17.86 ± 0.39

73.14 ± 3.78

23.06 ± 0.31

TT/22

132.45 ± 6.43b

130.30 ± 5.65

142.5 ± 5.85b

141.48 ± 13.08b

5.27 ± 0.46

23.93 ± 2.50

17.91 ± 0.43

71.73 ± 2.85

23.18 ± 0.50

P-value

0.030

0.116

0.015

0.042

0.680

0.891

0.761

0.205

0.281

g.162160146 T > C

TT/234

135.06 ± 5.30

132.76 ± 6.49

144.32 ± 3.67

150.90 ± 12.56

5.38 ± 0.50

24.15 ± 2.29

17.87 ± 0.38

72.99 ± 3.75

23.08 ± 0.39

TC/146

134.64 ± 4.78

132.33 ± 5.72

144.49 ± 5.05

151.54 ± 20.67

5.16 ± 0.37

23.96 ± 1.82

17.86 ± 0.36

72.63 ± 3.48

23.03 ± 0.35

CC/26

134.00 ± 4.01

131.81 ± 5.84

145.26 ± 5.37

151.52 ± 18.98

5.22 ± 0.42

24.65 ± 2.40

17.69 ± 0.47

72.54 ± 3.26

23.09 ± 0.33

P-value

0.495

0.665

0.308

0.987

0.034

0.315

0.075

0.591

0.185

g.162163775 T > G

TT/174

134.72 ± 4.78

132.41 ± 5.95

144.72 ± 5.13

150.97 ± 19.52ab

5.17 ± 0.38

23.94 ± 2.08

17.84 ± 0.38

72.84 ± 3.41

23.02 ± 0.31B

TG/183

135.03 ± 5.30

133.01 ± 6.25

145.30 ± 5.02

153.70 ± 16.01a

5.22 ± 0.42

24.20 ± 2.19

17.91 ± 0.36

73.08 ± 3.69

23.13 ± 0.38A

GG/46

134.57 ± 5.22

131.46 ± 6.76

144.25 ± 5.90

145.12 ± 27.43b

5.28 ± 0.46

24.24 ± 2.14

17.74 ± 0.44

71.92 ± 4.01

23.02 ± 0.26AB

P-value

0.786

0.284

0.362

0.022

0.206

0.453

0.021

0.155

0.005

g.162166224G > A

GG/150

134.84 ± 4.84

132.31 ± 6.47

144.80 ± 5.53

152.55 ± 16.03

5.25 ± 0.43

24.23 ± 2.06

17.80 ± 0.43b

72.52 ± 3.82

23.05 ± 0.37

GA/199

134.73 ± 5.32

132.48 ± 6.22

145.08 ± 4.78

152.29 ± 15.10

5.18 ± 0.39

23.99 ± 2.17

17.90 ± 0.33a

73.07 ± 3.38

23.09 ± 0.35

AA/57

135.25 ± 4.64

133.36 ± 5.21

144.72 ± 5.56

150.03 ± 24.56

5.23 ± 0.42

24.22 ± 2.28

17.84 ± 0.41ab

72.78 ± 3.88

23.07 ± 0.26

P-value

0.797

0.543

0.844

0.503

0.315

0.535

0.040

0.375

0.583

g.162166654 T > A

TT/150

134.84 ± 4.84

132.31 ± 6.47

144.8 ± 5.53

150.03 ± 24.56

5.25 ± 0.43

24.23 ± 2.06

17.80 ± 0.43b

72.52 ± 3.82

23.05 ± 0.37

TA/199

134.73 ± 5.32

132.48 ± 6.22

145.08 ± 4.78

152.29 ± 15.10

5.18 ± 0.39

23.99 ± 2.17

17.90 ± 0.33a

73.07 ± 3.38

23.09 ± 0.35

AA/57

135.25 ± 4.64

133.36 ± 5.21

144.72 ± 5.56

152.55 ± 16.03

5.23 ± 0.42

24.22 ± 2.28

17.84 ± 0.41ab

72.78 ± 3.88

23.07 ± 0.26

P-value

0.797

0.543

0.844

0.503

0.315

0.535

0.040

0.375

0.583

g.162167165C > A

CC/150

135.25 ± 4.64

132.31 ± 6.47

144.80 ± 5.53

150.03 ± 24.56

5.23 ± 0.42

24.23 ± 2.06

17.80 ± 0.43b

72.52 ± 3.82

23.05 ± 0.37

CA/199

134.73 ± 5.32

132.48 ± 6.22

145.08 ± 4.78

152.29 ± 15.10

5.18 ± 0.39

23.99 ± 2.16

17.90 ± 0.33a

73.07 ± 3.38

23.09 ± 0.35

AA/57

134.84 ± 4.84

133.36 ± 5.21

144.72 ± 5.56

152.55 ± 16.03

5.25 ± 0.43

24.22 ± 2.28

17.84 ± 0.41ab

72.78 ± 3.88

23.07 ± 0.26

P-value

0.797

0.543

0.844

0.503

0.315

0.535

0.040

0.375

0.583

g.162167314A > C

AA/150

134.84 ± 4.84

132.31 ± 6.47

144.80 ± 5.53

150.03 ± 24.56

5.25 ± 0.43

24.23 ± 2.06

17.80 ± 0.43b

72.52 ± 3.82

23.05 ± 0.37

AC/199

134.73 ± 5.32

132.48 ± 6.22

145.08 ± 4.78

152.29 ± 15.10

5.18 ± 0.39

23.99 ± 2.17

17.90 ± 0.33a

73.07 ± 3.38

23.09 ± 0.35

CC/57

135.25 ± 4.64

133.36 ± 5.21

144.72 ± 5.56

152.55 ± 16.03

5.23 ± 0.42

24.22 ± 2.28

17.84 ± 0.41ab

72.78 ± 3.88

23.07 ± 026

P-value

0.797

0.543

0.844

0.503

0.315

0.535

0.040

0.375

0.583

g.162172653G > C

GG/150

134.81 ± 4.85

132.24 ± 6.46

144.73 ± 5.42

149.77 ± 24.36

5.25 ± 0.43

24.23 ± 2.06

17.80 ± 0.43b

72.52 ± 3.83

23.05 ± 0.37

GC/200

134.71 ± 5.33

132.52 ± 6.22

145.08 ± 4.92

152.38 ± 15.33

5.18 ± 0.39

23.98 ± 2.17

17.90 ± 0.33a

73.07 ± 3.36

23.08 ± 0.34

CC/56

135.39 ± 4.54

133.44 ± 5.22

144.91 ± 5.42

152.92 ± 15.93

5.23 ± 0.43

24.26 ± 2.28

17.84 ± 0.42ab

72.79 ± 3.91

23.07 ± 0.26

P-value

0.671

0.466

0.821

0.379

0.296

0.475

0.037

0.367

0.588

g.162182976C > T

CC/195

134.65 ± 5.24

132.33 ± 6.20

144.64 ± 5.36

151.93 ± 15.65

5.22 ± 0.41

24.20 ± 2.24

17.87 ± 0.39

72.77 ± 3.58

23.09 ± 0.33

CT/177

134.88 ± 4.72

132.82 ± 6.11

145.38 ± 4.89

151.32 ± 23.04

5.18 ± 0.38

23.91 ± 2.02

17.85 ± 0.36

72.93 ± 3.51

23.03 ± 0.33

TT/33

135.77 ± 5.66

132.48 ± 6.57

144.06 ± 5.44

149.86 ± 16.55

5.36 ± 0.49

24.61 ± 2.08

17.82 ± 0.47

72.72 ± 4.47

23.18 ± 0.47

P-value

0.493

0.743

0.240

0.840

0.049

0.158

0.728

0.893

0.038

g.162216538G > A

GG/66

134.38 ± 4.59

131.76 ± 6.59

144.42 ± 5.26

151.79 ± 17.39

5.21 ± 0.41

24.03 ± 2.05

17.79 ± 0.41

72.08 ± 3.71b

23.00 ± 0.39

GA/124

134.59 ± 5.51

131.69 ± 6.06

144.72 ± 5.21

151.83 ± 14.82

5.20 ± 0.40

24.14 ± 2.07

17.84 ± 0.41

72.57 ± 3.40ab

23.04 ± 0.35

AA/168

135.20 ± 4.51

133.24 ± 5.57

145.12 ± 5.22

151.78 ± 20.14

5.19 ± 0.39

24.05 ± 2.09

17.90 ± 0.36

73.31 ± 3.59a

23.09 ± 0.31

P-value

0.406

0.530

0.614

1.000

0.927

0.887

0.115

0.037

0.157