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Table 1 Primer sequences, annealing temperature, and products size for Dezhou donkey PRKG2 gene

From: Polymorphism detection of PRKG2 gene and its association with the number of thoracolumbar vertebrae and carcass traits in Dezhou donkey

Primers/loci

Sequence 5′–3′

Annealing temperature (°C)

Products size (bp)

g.162153251G > A

F:GCACCAGGATACAGACA

62-52touchdown

418

 

R:CATAAACTGCCCTCACT

  

g.162156524C > T

F:TGTTAGGATACAGCGAGAA

62-52touchdown

818

 

R:CCACGATGGCAGAAACT

  

g.162158453C > T

F:CTACAACAATGCCCTCA

62-52touchdown

972

 

R:TGCTTACCACCTACCTC