Table 5 Primers used in the study
primers | (5′-3′)sequence of primers | purpose |
|---|---|---|
HcPKC-F | GCTTGTTTCCAGACTGACGA | Partial fragment amplifification of HcPKC |
HcPKC–R | CGTCAGTCTGGAAACAAGCAA | Partial fragment amplifification of HcPKC |
HcPKC-3’ | CCATCCATTCCTTGTAAACCTG | 3’RACE |
HcPKC-5’ | GTCAGTCTGGAAACAAGCAAAC | 5’RACE |
HcPKC-RT-F | AGTGAACGTGATGCAGAGGA | qPCR |
HcPKC-RT-R | GGTGTCAACACTGGCTTCTC | qPCR |
HcPKC-Y-F | AGTGAACGTGATGCAGAGGA | In situ hybridization |
HcPKC-Y-R | TAATACGACTCACTATAGGGGGTGTCAACACTGGCTTCTC | In situ hybridization |
EF1α-F | GGAACTTCCCAGGCAGACTGTGC | qPCR internal control |
EF1α-R | TCAAAACGGGCCGCAGAGAAT | qPCR internal control |